Abstract
Although cells of the dendritic cell [DC] lineage have been found in most tissues, only Langerhans cells and blood DCs have been examined for HIV-1 infection in vivo and in vitro. Reported levels of infection of blood DCs in vivo have ranged from high level infection with up to 20% of DCs infected1 to virtually undetectable in patients at similar stages of the disease2,3. A wide range of infectibility has also been found for blood DCs in vitro [see Cameron et al4 for review]. The differences may be at least partially attributed to the different isolation methods used and resultant phenotypic differences in the DC populations studied. Some studies have used DC-enriched populations mat contain only 30–75% DCs. In our hands, isolation methods that do not include specific depletion and cell sorting produce populations with unacceptable contamination with other cells including T cells. Dendritic cells are potent APCs and contamination with small numbers of T cells may assume a much greater significance than with less efficient APCs. DCs may induce the expansion of residual T cells and may be able to signal memory T cells to become productively infected without antigen recognition or overt stimulation and detectable proliferation5. For studies of DCs it is therefore critical to remove contaminating cells that may interact with the DCs, particularly when long term culture and p24 assays are used.
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Cameron, P.U., Lowe, M.G., Sotzik, F., Coughlan, A.F., Crowe, S.M., Shortman, K. (1995). Preferential Entry and Productive Infection of CD4 Expressing Lymphoid Dendritic Cells by Macrophage-Tropic HIV-1. In: Banchereau, J., Schmitt, D. (eds) Dendritic Cells in Fundamental and Clinical Immunology. Advances in Experimental Medicine and Biology, vol 378. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1971-3_96
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DOI: https://doi.org/10.1007/978-1-4615-1971-3_96
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