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Chlorophyll Triplet States in the CP47-D1-D2-Cytochrome b-559 Complex of Photosystem II

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The Photosynthetic Bacterial Reaction Center II

Part of the book series: Nato ASI Series ((NSSA,volume 237))

Abstract

Chlorophyll triplet states have been induced at cryogenic temperatures by illumination of isolated photosystem II CP47-Dl-D2-Cytochrome b-559 (CP47-RC) and D1-D2-Cytochrome b-559 (RC) complexes with repetitive laser flashes. The triplet induced in RC and RC-CP47 complexes had a lifetime of 1.6 ms (at 77K) and was characterized by an absorbance difference spectrum peaking at 680 nm. In CP47-RC complexes, an antenna triplet on CP47 was found with a lifetime of 0.7 ms; the quantum yield of radical pair triplet formation was about 0.2–0.3 below 100K and decreased at higher temperatures. The temperature dependence was analyzed with an equilibrium model for the chlorophyll excited states and the radical pair; the free energy change (ΔG) for charge separation was found to be about -50 meV and independent of temperature.

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References

  1. D.F. Ghanotakis and C.F. Yocum, Photosystem II and the oxygen-evolving complex, Ann. Rev. Plant Physiol. Plant Mol. Biol. 41:255 (1990)

    Article  CAS  Google Scholar 

  2. P.J. van Leeuwen, M.C. Nieveen, E. J. van de Meent, J.P. Dekker and H.J. van Gorkom, Rapid and simple isolation of pure photosystem II core and reaction center complexes from spinach, Photosynth. Res. 28:149 (1991)

    Article  Google Scholar 

  3. R. Barbato, H.L. Race, G. Friso and J. Barber, Chlorophyll levels in the pigment-binding proteins of photosystem II FEBS Lett. 286:86 (1991)

    Article  PubMed  CAS  Google Scholar 

  4. G.H. Schatz, H. Brock and A.R. Holzwarth, Picosecond kinetics of fluorescence and absorbance changes in photosystem II particles excited at low photon density, Proc. Natl. Acad. Sci. USA 84:8414 (1987)

    Article  PubMed  CAS  Google Scholar 

  5. S. Gerken, K. Brettel, E. Schlodder and H.T. Witt, Optical characterization of the immediate electron donor to chlorophyll aII + in O2-evolving photosystem II complexes. Tyrosine as possible electron carrier between chlorophyll aII and the water-oxidizing manganese complex, FEBS Lett. 237:69 (1988)

    Article  CAS  Google Scholar 

  6. G.H. Schatz, H. Brock and A.R. Holzwarth, Kinetic and energetic model for the primary processes in photosystem II Biophys. J. 54:397 (1988)

    Article  PubMed  CAS  Google Scholar 

  7. W. Leibl, J. Breton, J. Deprez and H.-W. Trissl, Photoelectric study on the kinetics of trapping and charge stabilization in oriented PS II membranes, Photosynth. Res. 22:257 (1989)

    Article  CAS  Google Scholar 

  8. F.J.E. van Mieghem, W. Nitschke, P. Mathis and A.W. Rutherford, The influence of the quinone-iron electron acceptor complex on the reaction centre photochemistry of photosystem II, Biochim. Biophys. Acta 977:207 (1989)

    Article  Google Scholar 

  9. F.J.E. van Mieghem, G.F.W. Searle, A.W. Rutherford and T.J. Schaafsma, The influence of the double reduction of QA on the fluorescence decay kinetics of photosystem II, Biochim. Biophys. Acta 1100:198 (1992)

    Article  Google Scholar 

  10. O. Nanba and K. Satoh, Isolation of a photosystem II reaction center consisting of D-l and D-2 polypeptides and cytochrome b-559, Proc. Natl. Acad. Sci. USA 84:109 (1987)

    Article  PubMed  CAS  Google Scholar 

  11. J.P. Dekker, N.R. Bowlby and C.F. Yocum CF, Chlorophyll and cytochrome b-559 content of the photochemical reaction center of photosystem II, FEBS Lett. 254:150 (1989)

    Article  CAS  Google Scholar 

  12. J. Petersen, J.P. Dekker, N.R. Bowlby, D.F. Ghanotakis, C.F. Yocum and G.T. Babcock, EPR characterization of the CP47-Dl-D2-cytochrome b-559 complex of photosystem II, Biochemistry 29:3226(1990)

    Google Scholar 

  13. M.R. Wasielewski, D.G. Johnson, M. Seibert and Govindjee, Determination of the primary charge separation rate in isolated photosystem II reaction centers with 500-fs time resolution, Proc. Natl. Acad. Sci USA 86:524 (1989)

    Article  PubMed  CAS  Google Scholar 

  14. M.R. Wasielewski, D.G. Johnson, Govindjee, C. Preston and M. Seibert, Determination of the primary charge separation rate in photosystem II reaction centers at 15K, Photosynth. Res. 22:89 (1989)

    Article  CAS  Google Scholar 

  15. R. Jankowiak, D. Tang, G.J. Small and M. Seibert, Transient and persistent hole burning of the reaction center of photosystem II, J. Phys. Chem. 93:1649 (1989)

    Article  CAS  Google Scholar 

  16. D. Tang, R. Jankowiak, M. Seibert, C.F. Yocum and G.J. Small, Excited-state structure and energy-transfer dynamics of two different preparations of the reaction center of photosystem II: a hole burning study, J. Phys. Chem. 94:6519 (1990)

    Article  CAS  Google Scholar 

  17. D. Tang, R. Jankowiak, M. Seibert and G.J. Small, Effects of detergent on the excited state structure and relaxation dynamics of the photosystem II reaction center: a high resolution hole burning study, Photosynth. Res. 27 19 (1991)

    Article  CAS  Google Scholar 

  18. T.A. Roelofs, M. Gilbert, V.A. Shuvalov and A.R. Holzwarth, Picosecond fluorescence kinetics of the D1-D2-Cyt-b-559 photosystem II reaction center complex. Energy transfer and primary charge separation processes, Biochim. Biophys. Acta 1060:237 (1991)

    CAS  Google Scholar 

  19. J.R. Durrant, G. Hastings, Q. Hong, J. Barber, G. Porter and D.R. Klug, Determination of P680 singlet state lifetimes in photosystem two reaction centres, Chem. Phys. Lett. 188:54 (1992)

    Article  CAS  Google Scholar 

  20. T.A. Roelofs, S.L.S. Kwa, R. van Grondelle, J.P. Dekker and A.R. Holzwarth, Primary processes and structure of the photosystem II reaction center: II. Low temperature picosecond fluorescence kinetics of a Dl-D2-Cyt-b559 reaction center complex isolated by short Triton-exposure, Biochim. Biophys. Acta, submitted for publication

    Google Scholar 

  21. R.V. Danielius, K. Satoh, P.J.M. van Kan, J.J. Plijter, A.M. Nuijs and H.J. van Gorkom, The primary reaction of photosystem II in the Dl-D2-cytochrome b-559 complex, FEBS Lett. 213:241 (1987)

    Article  CAS  Google Scholar 

  22. Y. Takahashi, Ö. Hansson, P. Mathis and K. Satoh, Primary radical pair in the photosystem II reaction centre, Biochim. Biophys. Acta 893:49 (1987)

    Article  CAS  Google Scholar 

  23. P.J.M. van Kan, S.C.M. Otte, F.A.M. Kleinhcrenbrink, M.C. Nieveen, TJ. Aartsma and H.J. van Gorkom, Time-resolved spectroscopy at 10K of the photosystem II reaction center; deconvolution of the red absorption band, Biochim. Biophys. Acta 1020:146 (1990)

    Article  Google Scholar 

  24. Govindjee, M. van de Ven, C. Preston, M. Seibert and E. Gratton, Chlorophyll a fluorescence lifetime distributions in open and closed photosystem II reaction center preparations, Biochim. Biophys. Acta 1015:173 (1990)

    Google Scholar 

  25. P.J. Booth, B. Crystall, I. Ahmad, J. Barber, G. Porter and D.R. Klug, Observation of multiple radical pair states in photosystem 2 reaction centers, Biochemistry 30:7573 (1991)

    Article  PubMed  CAS  Google Scholar 

  26. M.Y. Okamura, K. Satoh, R.A. Isaacson and G. Feher, Evidence of the primary charge separation in the D1D2 complex of photosystem II from spinach: EPR of the triplet state, in: “Progress in Photosynthesis Research,” J. Biggins, ed., Mart in us Nijhoff Publishers, Dordrecht, The Netherlands, Vol. I, p. 379(1987)

    Google Scholar 

  27. J.R. Durrant, L.B. Giorgi, J. Barber, D.R. Klug and G. Porter, Characterisation of triplet states in isolated photosystem II reaction centres: oxygen quenching as a mechanism for photodamage, Biochim. Biophys. Acta 1017:167 (1990)

    Article  CAS  Google Scholar 

  28. S.L.S. Kwa, W.R. Newell, R. van Grondelle and J.P. Dekker, The reaction center of photosystem II studied with polarized fluorescence spectroscopy, Biochim. Biophys. Acta 1099:193 (1992)

    Article  CAS  Google Scholar 

  29. F. van Mourik, C.J.R. van der Oord, K.J. Visscher, P.S. Parkes-Loach, P.A. Loach, R.W. Visschers and R. van Grondelle, Exciton interactions in the light-harvesting antenna of photosynthetic bacteria studied with triplet-singlet spectroscopy and singlet-triplet annihilation on the B820 subunit form of Rhodospirillum rubrum. Biochim. Biophys. Acta 1059:111 (1991)

    Article  Google Scholar 

  30. A.J. Hoff, Triplets: phosphorescence and magnetic resonance, in: “Light Emission by Plants and Bacteria,” Govindjee, J. Amesz and D.C. Fork, eds. Academic Press, New York, p. 225 (1986)

    Google Scholar 

  31. J.P. Dekker, S.D. Betts, C.F. Yocum and E.J. Boekema, Characterization by electron microscopy of isolated particles and two-dimensional crystals of the CP47-Dl-D2-cytochrome b-559 complex of photosystem II, Biochemistry 29:3220 (1990)

    Article  PubMed  CAS  Google Scholar 

  32. P.J.M. van Kan, M.L. Groot, I.H.M. van Stokkum, S.L.S. Kwa, R. van Grondelle and J.P. Dekker, Chlorophyll triplet states in the CP47 core antenna protein of photosystem II, In Proc. IXth International Congress on Photosynthesis, Nagoya, Japan, in press

    Google Scholar 

  33. T.A. Roelofs and A.R. Holzwarth, In search of a putative long-lived relaxed radical pair state in closed photosystem II: kinetic modelling of picosecond fluorescence data, Biophys. J. 57:1143 (1990)

    Article  Google Scholar 

  34. P.J. Booth, B. Crystall, L.B. Giorgi, J. Barber, D.R. Klug and G. Porter, Thermodynamic properties in Dl/D2/cytochrome b-559 reaction centres investigated by time-resolved fluorescence spectroscopy, Biochim. Biophys. Acta 1016:141 (1990)

    Article  CAS  Google Scholar 

  35. N.W.T. Woodbury and W.W. Parson, Nanosecond fluorescence from isolated photosynthetic reaction centers of Rhodopseudomonas sphaeroides, Biochim. Biophys. Acta 767:345 (1984)

    Article  PubMed  CAS  Google Scholar 

  36. A. Ogrodnik, M. Volk and M.E. Michel-Bcyerle, On the energetics of the states 1P*, 3P* and P+H- in reaction centers of Rb. sphaeroides, in: “The Photosynthetic Bacterial Reaction Center. Structure and Dynamics,” J. Breton and A. Verméglio, eds., Plenum Press, New York, p. 177 (1988)

    Chapter  Google Scholar 

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Authors and Affiliations

  1. Department of Physics and Astronomy, Free University, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands

    Paul J. M. van Kan, Marloes L. Groot, Stefan L. S. Kwa, Jan P. Dekker & Rienk van Grondelle

Authors
  1. Paul J. M. van Kan
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  2. Marloes L. Groot
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  3. Stefan L. S. Kwa
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  4. Jan P. Dekker
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  5. Rienk van Grondelle
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Editor information

Editors and Affiliations

  1. CEN Saclay, Gif-sur-Yvette, France

    Jacques Breton

  2. CEN Cadarache, Saint Paul lez Durance, France

    André Verméglio

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© 1992 Springer Science+Business Media New York

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van Kan, P.J.M., Groot, M.L., Kwa, S.L.S., Dekker, J.P., van Grondelle, R. (1992). Chlorophyll Triplet States in the CP47-D1-D2-Cytochrome b-559 Complex of Photosystem II. In: Breton, J., Verméglio, A. (eds) The Photosynthetic Bacterial Reaction Center II. Nato ASI Series, vol 237. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3050-3_45

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  • DOI: https://doi.org/10.1007/978-1-4615-3050-3_45

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6330-9

  • Online ISBN: 978-1-4615-3050-3

  • eBook Packages: Springer Book Archive

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