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The Effect of Quantitative Ratio Between Primer Pairs on Pcr Products in Multi-Target Amplification

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Modern Applications of DNA Amplification Techniques
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Abstract

The enormous efforts spent in studying the PCR enable us to control it through its components and conditions mainly qualitatively and partly quantitatively1–7. This statement is valid for single fragment amplification by PCR.

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References

  1. Christodoulou K., P. Ioannou and L. Middleton. Molecular genetic detection of Xp21 muscular dystrophy carriers in Cyprus. Biomed Pharmacother, 48: 355–358 (1994).

    Article  PubMed  CAS  Google Scholar 

  2. Jeffrey S., R. Chamberlain, A. Gibbs, E. J. Ranier and C.T. Caskey. Multiplex PCR for the diagnosis of Duchenne Muscular Dystrophy. PCR protocols. Ed. Michael A. Innis. Academic Press, INC. Harcourt Brace Jovanovich, Publishers, p272–281 (1990).

    Google Scholar 

  3. Apostolakos M. J., W. H. Schuermann, M. W. Frampton, M. J. Utell and J. C. Willey. Measurement of gene expression by multiplex competitive polymerase chain reaction. Anal Biochem, 213:277–284 (1993).

    Article  PubMed  CAS  Google Scholar 

  4. Abbs S. and M. Bobrow. Analysis of quantitative PCR for the diagnosis of deletion and duplication carriers in the dystrophin gene. J Med Genet, 29: 191–196 (1992).

    Article  PubMed  CAS  Google Scholar 

  5. Palejwala V.A., R.W. Rzepka; D. Simha and M.Z. Humayun. Quantitative multiplex sequence analysis of mutational hot spots-Frequency and specificity of mutations induced by a site-specific ethenocytosine in M13 viral DNA. Biochemistry, 32:4105–4111 (19

    Article  PubMed  CAS  Google Scholar 

  6. Pertl B., U. Weitgasser, S. Kopp, P. M. Kroisel, J. Sherlock and M. Adinolfi. Rapid detection of trisomies 21 and 18 and sexing by quantitative fluorescent multiplex PCR. Hum Genet, 98: 55–59 (1996).

    Article  PubMed  CAS  Google Scholar 

  7. Mansfield E.S. Diagnosis of Down syndrome and other anaploidies using quantitative polymerase chain reaction and small tandem repeat polymorphisms. Hum Mol Genet, 2: 43–50 (1993).

    Article  PubMed  CAS  Google Scholar 

  8. Eggeling F.V., M. Freytag, R. Fashold, B. Horsthemke and U. Claussen. Rapid detection of trisomy 21 by quantitative PCR. Hum Genet, 91:567–570 (1993).

    Article  Google Scholar 

  9. Morrison C. and F. Gannon. The impact of the PCR plateau phase on quantitative PCR. Biochim Biophys Acta, 1219:493–498(1994).

    Article  PubMed  CAS  Google Scholar 

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© 1997 Springer Science+Business Media New York

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Bercovich, D., Regev, Z., Ratz, T., Plotsky, Y. (1997). The Effect of Quantitative Ratio Between Primer Pairs on Pcr Products in Multi-Target Amplification. In: Lassner, D., Pustowoit, B., Rolfs, A. (eds) Modern Applications of DNA Amplification Techniques. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5379-3_9

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  • DOI: https://doi.org/10.1007/978-1-4615-5379-3_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7455-8

  • Online ISBN: 978-1-4615-5379-3

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