Abstract
Single sodium channel currents were measured in cell-atached patches of native and chloramine-T treated neuroblastoma cells, N1E 115. Channels modified by chloramine-T can open and close several times during depolarization. The open time of these channels was never longer than that of the native channels. Therefore the slower decay of the averaged current must be explained by the flickering behaviour of the modified channels.
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References
E. Carbone, H. D. Lux, Sodium channels in cultured chick dorsal root ganglion neurones, Eur. Biophys. J. 13: 259 (1986).
D. Colquhoun, B. Sakmann, Fast events in single-channel currents activated by acetylcholine and its analogues at the frog muscle endplate J. Physiol. 369: 501 (1985).
D. Colquhoun, F. Sigworth, Fitting and statistical analysis of single channel records, In “Single Channel Recording”, B. Sakmann, E. Neher, eds, Plenum Press, New York, 191 (1983).
O. Hamill, A. Marty, E. Neher, B. Sakmann, F. Sigworth, Iiproved patch clamp techniques for high-resolution current recording from cells and cell-free membrane patches, Pflug. Arch. Eur. J. Physiol. 391: 85 (1981).
D. Hof, A pulse generating and data recording system based on the microcomputer PDP 11/23, Comput. Method. Program. Biomed. in press (1987).
H. Meves, N. Rubly, Kinetics of sodium current and gating current in the frog node of Ranvier, Pflug. Arch. 407: 18 (1986).
W. Moolenaar, I. Spector, Ionic currents in cultured mouse neuroblastoma cells under voltage clamp conditions, J. Physiol. 278: 265 (1978).
K. Nagy, J. Kiss, D. Hof, Single Na channels in mouse neuroblastoma membranes. Indication for two open states, Pflug. Arch. 399: 302 (1983).
J. Patlak, R. Horn, Effect of N-bromacetamide on single sodium channel currents in excised membrane patches, J. Gen. Physiol. 79: 333 (1982).
M. Rack, N. Rubly, C. Waschow, Effects of some chemical reagents on sodium inactivation in myelinated nerve fibres of the frog, Biophys. J. in press (1986).
J. Schmidtmayer, Behaviour of chemically modified sodium channels in frog nerve supports a three-state model of inactivation, Pflug. Arch 404: 21 (1985).
G. Wang, Irreversible modification of sodium channel inactivation in toad myelinated nerve fibres by the oxidant chloramine-T, J. Physiol (Lond) 346: 127 (1984).
G. Wang, M. Brodwick, D. Eaton, Removal of sodium channel inactivation in squid axon by oxidant chloramine-T, J. Gen. Physiol. 86: 289 (1985).
D. Yamamoto, J. Z. Yeh, Kinetics of 9-aminoacridine block of single sodium channels, J. Gen. Physiol. 84: 361 (1984).
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© 1988 Plenum Press, New York
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Iliev, I., Nagy, K. (1988). Effect of Chloramine-T on Single Sodium Channels in Neuroblastoma Cells. In: Markov, M., Blank, M. (eds) Electromagnetic Fields and Biomembranes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9507-6_32
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DOI: https://doi.org/10.1007/978-1-4615-9507-6_32
Publisher Name: Springer, Boston, MA
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