Abstract
We used intracerebral dialysis to monitor extracellular levels of dopamine and its major metabolites dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in the striatum of chloralose/urethane-anesthetized rats. Levels of these compounds were determined after intraperitoneal administration of phenylalanine (200, 500, and 1000-mg/kg). A dose of 200 mg phenylalanine per kg (which increases brain tyrosine by more than phenylalanine) increased basal dopamine release by 59%, peaking at 75 min. There was no change in basal dopamine release after the 500-mg dose, whereas the 1000-mg/kg dose (which increases brain phenylalanine more than tyrosine) significantly reduced (26%) dopamine release. No corresponding changes were observed in the concentrations of DOPAC and HVA with any of the treatments, indicating that changes in brain phenylalanine and tyrosine levels may selectively affect production of the dopamine molecules that are preferentially released into synapses.
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References
Delgado, J.M.R., De Feudis, F.V., Roth, R.H., Ryugo, D.K., and Mitruka, B.M. (1972). Dialytrode for long term intracerebral perfusion in awake monkeys. Arch. Int. Pharmacodyn. Ther. 198:9.
Fernstrom, J.D., and Faller, D.V. (1978). Neutral amino acids in the brain: changes in response to food ingestion. J. Neurochem. 30:1531–1538.
Gibson, C.J., Deikel, S.M., Young, S.N., and Binik, Y.M. (1982). Behavioural and biochemical effects of tryptophan, tyrosine and phenylalanine in mice. Psychopharmacology 76:118–121.
Ikeda, M., Levitt, M., and Udenfriend, S. (1967). Phenylalanine as substrate and inhibitor of tyrosine hydroxylase, Arch. Biochem. Biophys. 120:420–427.
Katz, I., Lloyd, T., and Kaufman, S. (1976). Studies on phenylalanine and tyrosine hydroxylation by rat brain tyrosine hydroxylase. Biochim. Biophys. Acta 445:567–578.
Krause, W., Halminski, M., McDonald, L., Dembure, P., Salvo, R., Friedes, D., and Elsas, L. (1985). Biochemical and neuropsychological effects of elevated plasma phenylalanine in patients with treated phenylketonuria. J. Clin. Invest. 75:40–48.
Maher, T., and Wurtman, R.J. (1987). Possible neurological effects of aspartame, a widely used food additive. In Environmental health perspectives, Twentieth anniversary of the National Institute of Environmental Health Sciences 75:53–58.
McKean, C.M. (1972). The effects of high phenylalanine concentrations on serotonin and catecholamine metabolism in the human brain. Brain Res. 47:469–476.
Milner, J.D., Irie, K., and Wurtman, R.J. (1986). Effects of phenylalanine on the release of endogenous dopamine from rat striatal slices. J. Neurochem. 47:1444–1448.
Nadler, H.L., and Hsia, D.Y. (1961). Epinephrine metabolism in phenylketonuria. Proc. Soc. Exp. Biol. 107:721–723.
Oldendorf, W.H. (1973). Stereospecificity of the blood brain barrier to amino acids. Am. J. Physiol. 224:967–969.
Pardridge, W. (1977). Regulation of amino acid availability to the brain. In Wurtman, R.J., and Wurtman, J.J. (eds.), Nutrition and the brain, Vol. 1. New York: Raven Press, pp. 142–204.
Paxinos, G. and Watson, C. (1982). The rat brain in stereotaxic coordinates. New York: Academic Press.
Pinto, J.M.B. and Maher, T.J. (1988). Aspartame administration potentiates fluorothyl-and pentylenetetrazole-induced seizure in mice. Neuropharmacol. 27:51–5.
Sharp, T., Zetterstrom, T., and Ungerstedt, U. (1986). An in vivo study of dopamine release and metabolism in rat brain regions using intracerebral dialysis. J. Neurochem. 47:113–122.
Thurmond J.B., Kramacy, N.R., Lasley, S.M., and Brown, J.W. (1980). Dietary amino acid precursors: effect on central monoamines, aggression and locomotor activity in the mouse. Pharmacol. Biochem. Behav. 12:525–532.
Ungerstedt, U., Herrera-Marschitz, M., Jungnelius, U., Stahle, L., Tossman, U., and Zetterstrom, T. (1982). Dopamine synaptic mechanisms reflected in studies combining behavioural recordings and brain dialysis. In Kohsaka, M. et al. (eds.), Advances in the biosciences, Vol. 37: Advances in dopamine research. Oxford: Pergamon Press, pp. 219–231.
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© 1988 Birkhäuser Boston
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During, M.J., Acworth, I.N., Wurtman, R.J. (1988). An In Vivo Study of Dopamine Release in Striatum: The Effects of Phenylalanine. In: Wurtman, R.J., Ritter-Walker, E. (eds) Dietary Phenylalanine and Brain Function. Birkhäuser Boston. https://doi.org/10.1007/978-1-4615-9821-3_8
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DOI: https://doi.org/10.1007/978-1-4615-9821-3_8
Publisher Name: Birkhäuser Boston
Print ISBN: 978-1-4615-9823-7
Online ISBN: 978-1-4615-9821-3
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