Abstract
The binding of α- and β-methyl-D-glucopyranoside and β-(o-iodopheryl)-D-glucopyranoside to concanavalin A has been studied by carbon-13 nuclear magnetic resonance techniques. The kinetics and binding orientations of these saccharides relative to the transition metal ion site in the protein have been determined.
This work was supported by U. S. P. H. S. Grants No. GM-00065 and AI-05336-11 and the American Cancer Society Grant No. IC-61D.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Abragam, A. (1961). Principles of Nuclear Magnetism, New York, N. Y., Oxford University Press, Chapt. 8.
Brewer, C. F., Sternlicht, H., Marcus, D. M., and Grollman, A. P. (1973). In Lysozyme. Osserman, E., Beychok, S., and Canfield, R., Eds., New York, N. Y., Academic Press. In press.
Brewer, C. F., Sternlicht, H., Marcus, D. M., and Grollman, A. P. (1973). “Binding of 13C-enriched a-methyl-D-glucopyranoside to concanavalin A as studied by carbon magnetic resonance.” Proc. Nat. Acad. Sci. U. S. A. 70, 1007.
Brewer, C. F., Sternlicht, H., Marcus, D. M., and Grollman, A. P. (1973b) “Interactions of sachharides with concanavalin A. Mechanism of binding of a-and 6-methyl-D-glucopyrannoside to concanavalin A as determined by 13C nuclear magnetic resonance.” Biochemistry 12, 4448.
Edelman, G. M., Cunningham, B. A., Reeke, G. M., Jr., Becker, J. W., Waxdal, M. J., and Wang, J. L. (1972). “The covalent and three-dimensional structure of concanavalin A.” Proc. Nat. Acad. Sci. U. S. A. 69, 2580.
Goldstein, I. J., Hollerman, C. E., and Smith, E. E. (1965). “Protein-carbohydrate interaction. II. Inhibition studies on the interaction of concanavalin A with polysaccharides.” Biochemistry 4, 876.
Gray, R. D., and Glew, R. H. (1973). “The kinetics of carbohydrate binding to concanavalin A.” J. Biol. Chem. 248, 7547.
Hardman, K. D., and Ainsworth, C. F. (1973). “Binding of non-polar molecules by crystalline concanavalin A.” Biochemistry 12, 4442.
Inbar, M., and Sachs, L. (1969). “Interaction of the carbohydrate-binding protein concanavalin A with normal and transformed cells.” Proc. Nat. Acad. Sci. U. S. A. 63, 1418.
Sharon, N., and Lis, H. (1972). “Lectins: Cell-agglutinating and sugar-specific proteins.” Science 177, 949.
Shoham, J., Inbar, M., and Sachs, L. (1970). “Differential toxicity on normal and transformed cells in vitro and inhibition of tumor development in vivo by concanavalin A.” Nature 227, 1244.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1975 Plenum Press, New York
About this chapter
Cite this chapter
Brewer, C.F., Sternlicht, H., Marcus, D.M., Grollman, A.P. (1975). 13C NMR Studies of the Interaction of Concanavalin A with Saccharides. In: Chowdhury, T.K., Weiss, A.K. (eds) Concanavalin A. Advances in Experimental Medicine and Biology, vol 55. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0949-9_4
Download citation
DOI: https://doi.org/10.1007/978-1-4684-0949-9_4
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-0951-2
Online ISBN: 978-1-4684-0949-9
eBook Packages: Springer Book Archive