Abstract
During an 8-yr period, 862 stool specimens from patients with gastroenteritis were examined by electron microscopy after negative staining with 2% phosphotungstic acid (pH 6.5) (1). Forty-one percent of the specimens submitted over an 8-yr period were determined to be positive for virus or viruslike particles belonging to one or more of seven morphologically distinct viral groups. Coronaviruslike particles (CVLPs) were present in 69.8% of the positive stool specimens. Membranous profiles containing “complement-type” holes (10 nm in diameter) were identified in some preparations containing CVLPs. The second most prevalent viral agent found in stool specimens was the rotavirus (17% of all positive stools). The incidence of other viruses identified in the survey were as follows: adenovirus 4.5%, picorna/parvovirus agents 2.9%, Norwalk-like agent 2.9%, astrovirus 1.9%, and calicivirus 0.5%. Unclassified small round viruses (~25–30 nm in diameter) represented 0.5%. It was also determined that there was a seasonal distribution in excretion of all viruses except for CVLPs. A greater number of viruses were identified in the cooler, drier months of the year.
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C.M. Payne, C.G. Ray, V. Borduin, L.L. Minnich, and M.D. Lebowitz, An eight-year study of the viral agents of acute gastroenteritis in humans: ultrastructural observations and seasonal distribution with a major emphasis on coronavirus-like particles. Diag Micro-biol Infect Dis 5: 39–54 (1986).
Y.E. Vaucher, C.G. Ray, L.L. Minnich, CM. Payne, D. Beck, and P. Lowe, Pleomorphic, enveloped, virus-like particles associated with gastrointestinal illness in neonates. J Infect Dis 145: 27–36 (1982).
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© 1987 Plenum Press, New York
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Payne, C.M., Ray, C.G., Borduin, V., Minnich, L.L., Lebowitz, M.D. (1987). An Eight-Year Study of the Viral Agents of Acute Gastroenteritis in Humans: Ultrastructural Observations and Seasonal Distribution with A Major Emphasis on Coronavirus-like Particles. In: Lai, M.M.C., Stohlman, S.A. (eds) Coronaviruses. Advances in Experimental Medicine and Biology, vol 218. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1280-2_74
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DOI: https://doi.org/10.1007/978-1-4684-1280-2_74
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