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Physicochemical Characterization of Low Density Lipoproteins

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Low Density Lipoproteins

Abstract

The principal reason for the application of physical or physicochemical methods to the investigation of lipoproteins is to lead to an understanding of the way in which the various, chemically quite different components, proteins, phospholipids, cholesterol, cholesteryl esters, and triglycerides, combine to form discrete macromolecular complexes. The knowledge of the phenomena involved in this assembly is fundamental to a further understanding of the role played by these complexes in the lipid transport of living organisms and of the way in which these structures can perform their functions. Furthermore, in addition to the metabolic function, the structure may also contain valuable information about the catabolic fate of these important macromolecules.

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References

  1. T. Forte, and A. V. Nichols. 1972. Application of electron microscopy to the study of plasma lipoprotein structure. Adv. Lipid Res. 10:1–41.

    PubMed  CAS  Google Scholar 

  2. S. Margolis. 1969. Structure of very low and low density lipoproteins. In: Structural and Functional Aspects of Lipoproteins in Living Systems. Ed. by E. Tria and A. M. Scanu. Academic Press, London, pp. 369–424.

    Google Scholar 

  3. R. M. C. Dawson. 1968. The nature of the interaction between protein and lipid during the formation of lipoprotein membranes In: Biological Membranes. Physical Fact and function. Ed. by D. Chapman. Academic Press, New York, pp. 203–232.

    Google Scholar 

  4. S. J. Singer. 1971. The molecular organization of biological membranes. In: Structure and Function of Biological Membranes. Ed. by L. I. Rothfield. Academic Press, New York, pp. 145–222.

    Google Scholar 

  5. C. Tanford. 1973. The Hydrophobic Effect: Formation of Micelles and Biological Membranes. John Wiley and Sons, New York.

    Google Scholar 

  6. D. Chapman. 1969. The physico-chemical approach to the study of lipoprotein interactions. In: Structural and Functional Aspects of Lipoproteins in Living Systems. Ed. by E. Tria and A. M. Scanu. Academic Press, London, pp. 3–36.

    Google Scholar 

  7. K. O. Pedersen. 1945. Ultracentrifugal Studies on Serum and Serum Fractions. Almquist and Wiksells, Uppsala.

    Google Scholar 

  8. G. H. Adams and V. N. Schumaker. 1969. Rapid molecular weight estimation for low density lipoproteins. Anal. Biochem. 29:117–129.

    Article  PubMed  CAS  Google Scholar 

  9. W. R. Fisher, M. E. Granade, and J. L. Mauldin. 1971. Hydrodynamic studies of human low density lipoproteins. Evaluation of the diffusion coefficient and the preferential hydration. Biochemistry 10:1622–1629.

    Article  PubMed  CAS  Google Scholar 

  10. H. K. Schachman. 1957. Ultracentrifugation, diffusion and viscosimetry. Methods Enzymol. 4:32–103.

    Article  Google Scholar 

  11. V. N. Schumaker. 1973. Hydrodynamic analysis of human low density lipoproteins. Acc. Chem. Res. 6:398–403.

    Article  CAS  Google Scholar 

  12. G. H. Adams and V. N. Schumaker. 1969. Polydispersity of human low density lipoproteins. Ann. N.Y. Acad. Sci. 164:130–146.

    Article  PubMed  CAS  Google Scholar 

  13. W. R. Fisher, M. E. Granade, M. Hammond, and G. L. Warmke. 1972. Measurements of the molecular weight variability of plasma low density lipoproteins among normals and subjects with hyper-β-lipoproteinemia. Demonstration of macromolecular heterogeneity. Biochemistry 11:519–525.

    Article  PubMed  CAS  Google Scholar 

  14. M. G. Hammond and W. R. Fisher. 1971. Characterization of a discrete series of low density lipoproteins in the disease hyper-pre-β-lipoproteinemia. Implications relating to the structure of plasma lipoproteins. J. Biol. Chem. 246:5454–5465.

    PubMed  CAS  Google Scholar 

  15. D. M. Lee and P. Alaupovic. 1974. Physicochemical properties of low-density lipoproteins of normal human plasma. Evidence for the occurence of lipoprotein B in associated and free forms. Biochem. J. 137:155–167.

    PubMed  CAS  Google Scholar 

  16. P. Alaupovic. 1972. Classification system of plasma lipoproteins. In: Protides of the Biological Fluids. Ed. by H. Peeters. Elsevier, Amsterdam, pp. 9–19.

    Google Scholar 

  17. R. W. De Blois, E. E. Uzgiris, S. K. Devi, and A. M. Gotto, Jr. 1973. Application of laser self-beat spectroscopic technique to the study of solutions of human plasma lowdensity lipoprotein. Biochemistry 12:2645–2649.

    Article  Google Scholar 

  18. F. T. Lindgren, L. C. Jensen. R. D. Wills, and N. K. Freeman. 1969. Flotation rates, molecular weights, and hydrated densities of the low-density lipoproteins. Lipids 4:337–344.

    Article  PubMed  CAS  Google Scholar 

  19. E. C. Gong, G. M. Forte, and A. V. Nichols. 1970. Properties of low and high density human serum lipoproteins following dehydration and rehydration procedures: Effect of sodium phosphotungstate. Physiol. Chem. Phys. 2:180–188.

    CAS  Google Scholar 

  20. A. M. Gotto, R. I. Levy, and D. S. Fredrickson. 1968. Preparation and properties of an apoprotein derivative of human serum β-lipoprotein. Lipids 3:463–470.

    Article  PubMed  CAS  Google Scholar 

  21. G. M. Forte, A. V. Nichols, and R. M. Glaeser. 1968. Electron microscopy of human serum lipoprotein using negative staining. Chem. Phys. Lipids 2:396–408.

    Article  CAS  Google Scholar 

  22. T. Forte, K. R. Norum, J. A. Glomset, and A. V. Nichols. 1971. Plasma lipoproteins in familial lecithin: cholesterol acyltransferase deficiency: Structure of low-and high-density lipoproteins as revealed by electron microscopy. J. Clin. Invest. 50:1141–1148.

    Article  PubMed  CAS  Google Scholar 

  23. H. B. Pollard and S. K. Devi. 1971. Construction of a three-dimensional isodensity map of the low density lipoprotein particle from human serum. Biochem. Biophys. Res. Commun. 44:593–599.

    Article  PubMed  CAS  Google Scholar 

  24. H. Pollard, A. M. Scanu, and E. W. Taylor. 1969. Geometrical arrangement of the protein subunits of human serum low-density lipoprotein: Evidence for a dodecahedral model. Proc. Natl. Acad. Sci. U.SA. 64:304–310.

    Article  CAS  Google Scholar 

  25. R. L. Hamilton, R. J. Havel, J. P. Kane, A. E. Blaurock, and T. Sata. 1971. Cholestasis: Lamellar structure of the abnormal human serum lipoprotein. Science 172:475–478.

    Article  PubMed  CAS  Google Scholar 

  26. O. Kratky. 1963. X-ray small angle scattering with substances of biological interest in diluted solution. Prog. Biophys. 13:107–173.

    Article  Google Scholar 

  27. O. Kratky and I. Pilz. 1972. Recent advances in applications of diffuse X-ray smallangle scattering on biopolymers in dilute solutions. Q. Rev. Biophys. 5:481–537.

    Article  PubMed  CAS  Google Scholar 

  28. P. Laggner, O. Kratky, G. Kostner, J. Sattler, and A. Holasek. 1972. Small angle X-ray scattering of LpA, the major lipoprotein family of human plasma high density lipoprotein HDL3. FEBS Lett. 27:53–57.

    Article  PubMed  CAS  Google Scholar 

  29. P. Laggner, K. Müller, O. Kratky, G. Kostner and A. Holasek. 1973. Studies on the structure of lipoprotein A of human high density lipoprotein HDL3: The spherically averaged electron density distribution. FEBS Lett. 33:77–80.

    Article  PubMed  CAS  Google Scholar 

  30. G. G. Shipley, D. Atkinson, and A. M. Scanu. 1972. Small-angle X-ray scattering of human serum high density lipoproteins. J. Supramol. Struct. 1:98–104.

    Article  PubMed  CAS  Google Scholar 

  31. K. Müller, P. Laggner, O. Kratky, G. Kostner, A. Holasek, and O. Glatter. 1974. X-ray small angle scattering of human plasma high density lipoprotein LpA from HDL2: Application of a new evaluation method. FEBS Lett. 40:213–218.

    Article  PubMed  Google Scholar 

  32. D. Atkinson, M. A. F. Davis, and R. B. Leslie. 1974. The structure of a high density lipoprotein (HDL3) from porcine plasma. Proc. R. Soc. London, Ser B. 186:165–180.

    Article  CAS  Google Scholar 

  33. L. Mateu, A. Tardieu, V. Luzzati, L. Aggerbeck, and A. M. Scanu. 1972. On the structure of human serum low density lipoprotein. J. Mol. Biol. 70:105–116.

    Article  PubMed  CAS  Google Scholar 

  34. M. J. Janiak, C. R. Loomis, G. G. Shipley, and D. M. Small. 1974. The ternary phase diagram of lecithin, cholesteryl linolenate and water: Phase behaviour and structure. J. Mol. Biol. 86:325–339.

    Article  PubMed  CAS  Google Scholar 

  35. A. M. Scanu and A. Tardieu. 1971. Temperature transitions of lipid mixtures containing cholesterol esters. Relevance to the structural problem of serum high density lipoprotein. Biochim. Biophys. Acta 231:170–174.

    PubMed  CAS  Google Scholar 

  36. P. Laggner, K. Müller, O. Kratky, G. Kostner, and A. Holasek. 1975. X-ray small angle scattering on human plasma lipoproteins. J. Colloidal Interface Sci. 55:102–108.

    Article  Google Scholar 

  37. R. J. Deckelbaum, G. G. Shipley, D. M. Small, R. S. Lees, and P. K. George. 1975. Thermal transitions in human plasma low density lipoproteins. Science 190:392–394.

    Article  PubMed  CAS  Google Scholar 

  38. W. Schmatz, T. Springer, J. Schelten, and K. Ibel. 1974. Neutron small-angle scattering: Experimental techniques and applications. J. Appl. Crystallogr. 7:96–116.

    Article  CAS  Google Scholar 

  39. D. Chapman and G. M. Dodd. 1971. Physicochemical probes of membrane structure. In: Structure and Function of Biological Membranes. Ed. by L. I. Rothfield. Academic Press, New York, pp. 13–81.

    Google Scholar 

  40. A. F. Horwitz. 1972. Nuclear magnetic resonance studies on phospholipids and membranes. In: Membrane Molecular Biology. Ed. by C. F. Fox and A. D. Keith. Sinauer Associates Inc., Stamford, Connecticut, pp. 164–191.

    Google Scholar 

  41. R. J. Mehlhorn and A. D. Keith. 1972. Spin labeling of biological membranes. In: Membrane Molecular Biology. Ed. by C. F. Fox and A. D. Keith. Sinauer Associates Inc., Stamford, Connecticut, pp. 192–227.

    Google Scholar 

  42. P. A. Jost, A. S. Waggoner, and O. H. Griffith. 1971. Spin labeling and membrane structure. In: Structure and Function of Biological Membranes. Ed. by L. I. Rothfield. Academic Press, New York, pp. 83–144.

    Google Scholar 

  43. J. M. Steim, O. J. Edner, and F. G. Bargoot. 1968. Structure of human serum lipoprotein. Nuclear magnetic resonance supports a micellar model. Science 162:909–911.

    Article  PubMed  CAS  Google Scholar 

  44. R. B. Leslie, D. Chapman, and A. M. Scanu. 1969. Nuclear magnetic resonance studies of serum low density lipoprotein (LDL2). Chem. Phys. Lipids 3:152–158.

    Article  PubMed  CAS  Google Scholar 

  45. E. G. Finer, R. Henry, R. B. Leslie, and R. N. Robertson. 1975. NMR studies of pig low-and high-density serum lipoproteins. Molecular motions and morphology. Biochim. Biophys. Acta 380:320–337.

    PubMed  CAS  Google Scholar 

  46. J. A. Hamilton, C. Talkowsky, R. F. Childers, E. Williams, A. Allerhand, and E. H. Cordes. 1974. Rotational and segmental motions in the lipids of human plasma lipoproteins. J. Biol. Chem. 249:4872–4875.

    PubMed  CAS  Google Scholar 

  47. G. Assmann, E. A. Sokolski, and H. B. Brewer, Jr. 1974. 31P nuclear magnetic resonance spectroscopy of native and recombined lipoproteins. Proc. Natl. Acad. Sci. U.S.A. 71:549–553.

    Article  PubMed  CAS  Google Scholar 

  48. R. B. Leslie. 1971. Some physical and physicochemical approaches to the structure of serum high density lipoproteins (HDL). In: Plasma Lipoproproteins. Biochemical Society Symposium No. 33. University College, London, April, 1971. Ed. by R. M. S. Smellie. Academic Press, New York, pp. 47–85.

    Google Scholar 

  49. A. D. Keith, R. J. Mehlhorn, N. K. Freeman, and A. V. Nichols. 1973. Spin-labeled lipid probes in serum lipoproteins. Chem. Phys. Lipids 10:223–236.

    Article  PubMed  CAS  Google Scholar 

  50. R. J. M. Smith and C. Green. 1974. Fluorescence studies of protein-sterol relationships in human plasma lipoproteins. Biochem. J. 137:413–415.

    PubMed  CAS  Google Scholar 

  51. S. Ghosh, M. K. Basu, and J. S. Schweppe. 1974. Interaction of 1-anilino-8-naphtalene sulfonate with human LDL. Biochim. Biophys. Acta 337:395–403.

    PubMed  CAS  Google Scholar 

  52. J. R. Brocklehurst, R. B. Freedman, D. J. Hancock, and G. K. Radda. 1970. Membrane studies with polarity-dependent excimer-forming fluorescent probes. Biochem. J. 116:721–731.

    PubMed  CAS  Google Scholar 

  53. R. A. Badley. 1975. The location of protein in serum low density lipoproteins: A fluorescence quenching study. Biochim. Biophys. Acta 379:517–528.

    PubMed  CAS  Google Scholar 

  54. G. Holzwarth. 1972. Ultraviolet spectroscopy of biological membranes. In: Membrane Molecular Biology. Ed. by C. F. Fox and A. D. Keith. Sinauer Associates Inc., Stamford, Connecticut, pp. 228–286.

    Google Scholar 

  55. A. M. Scanu and R. Hirz. 1968. Human serum low-density lipoprotein protein: Its conformation studied by circular dichroism. Nature 218:200–201.

    Article  PubMed  CAS  Google Scholar 

  56. A. M. Scanu, R. Pollard, R. Hirz, and K. Kothary. 1969. Conformational instability of human serum low-density lipoprotein: Effect of temperature. Proc. Natl. Acad. Sci. U.S.A. 62:171–178.

    Article  PubMed  CAS  Google Scholar 

  57. A. M. Gotto, R. I. Levy, and D. S. Fredrickson. 1968. Conformation of human β-lipoprotein: Evidence for the occurrence of beta structure. Proc. Natl. Acad. Sci. U.S.A. 60:1436–1441.

    Article  PubMed  CAS  Google Scholar 

  58. A. M. Gotto. 1969. Recent studies on the structure of human serum low-and highdensity lipoproteins. Proc. Natl. Acad. Sci. U.S.A. 64:1119–1127.

    Article  PubMed  CAS  Google Scholar 

  59. G. D. Dearborn and D. B. Wetlaufer. 1969. Reversible thermal conformation changes in human serum low-density lipoprotein. Proc. Natl. Acad. Sci. U.S.A. 62:179–185.

    Article  PubMed  CAS  Google Scholar 

  60. G. V. Kobozev and G. V. Troitskii. 1967. The conformation of β-lipoprotein of human blood. Mol. Biol. 1:202–213.

    CAS  Google Scholar 

  61. G. Chi Chen and J. P. Kane. 1974. Contribution of carotenoids to the optical activity of human serum low-density lipoprotein. Biochemistry 13:3330–3335.

    Article  PubMed  CAS  Google Scholar 

  62. H. B. Pollard and R. F. Chen. 1973. Fluorescence and CD studies on human serum LDL particles and lipid depleted derivatives. J. Supramol. Struct. 1:177–184.

    Article  PubMed  CAS  Google Scholar 

  63. S. E. Lux, R. Hirz, R. I. Shrager, and A. M. Gotto. 1972. The influence of lipid on the conformation of human plasma high density apolipoproteins. J. Biol. Chem. 247:2598–2606.

    PubMed  CAS  Google Scholar 

  64. J. Gwynne, B. Brewer, Jr., and H. Edelhoch. 1973. The molecular properties of apo A-I from human high density lipoprotein. J. Biol. Chem. 249:2411–2416.

    Google Scholar 

  65. A. Darke, E. G. Finer, A. G. Flook, and M. C. Phillips. 1972. Nuclear magnetic resonance study of lecithin-cholesterol interactions. J. Mol. Biol. 63:265–279.

    Article  PubMed  CAS  Google Scholar 

  66. M. C. Phillips and E. G. Finer. 1974. The stoichiometry and dynamics of lecithincholesterol clusters in bilayer membranes. Biochim. Biophys. Acta 356:199–206.

    Article  PubMed  CAS  Google Scholar 

  67. J. P. Segrest, R. L. Jackson, J. D. Morrisett, and A. M. Gotto, Jr. 1974. A molecular theory of lipid-protein interactions in the plasma lipoproteins. FEBS Lett. 38:247–253.

    Article  PubMed  CAS  Google Scholar 

  68. C. E. Day and R. S. Levy. 1969. Molecular structure of serum lipoproteins. J. Theor. Biol. 23:387–399.

    Article  PubMed  CAS  Google Scholar 

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© 1976 Plenum Press, New York

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Laggner, P. (1976). Physicochemical Characterization of Low Density Lipoproteins. In: Day, C.E., Levy, R.S. (eds) Low Density Lipoproteins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2250-4_2

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  • DOI: https://doi.org/10.1007/978-1-4684-2250-4_2

  • Publisher Name: Springer, Boston, MA

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