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Spin-Label Studies of Membranes

  • Chapter
Methods in Membrane Biology

Abstract

The development of electron paramagnetic resonance (EPR) spectroscopy preceded by more than a decade its application to a class of experiments referred to generally as spin-labeling. During this early period, the theoretical basis for the paramagnetic resonance spectra of numerous organic free radicals was established. These theoretical principles were applied to the determination of the structure and properties of paramagnetic species which occur naturally or are produced chemically or physically in biological materials. The paramagnetic species studied included intermediates in photosynthesis, biochemical reactions of metalloproteins, and free radicals in radiation-damaged tissues. Anisotropies in the EPR spectral parameters of organic free radicals were found to be of the order of a few tenths to a few hundred megahertz (MHz = 106 cps). The magnitude of these anisotropies suggested that spectral changes should be observed for organic free radicals with molecular motions occurring in the range from about 10-6 to 10-9 s. (The relation between spectral anisotropy and the effects of motion is discussed in more detail in section 3.3.) Because the rotational correlation times τ e (where τ e = 4πηr 3 /(3kT) for spherical molecules with radius r) for molecules, in aqueous solutions, of 1000 to 1,000,000 daltons fall within this time scale, problems involving the rotational motion of macromolecules are among those obviously amenable to solution by paramagnetic resonance spectroscopy.

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Authors and Affiliations

  1. Department of Chemistry, Johns Hopkins University, Baltimore, Maryland, USA

    Betty Jean Gaffney & Shaw-Chen Chen

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  1. Betty Jean Gaffney
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  2. Shaw-Chen Chen
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Editors and Affiliations

  1. Laboratory of Cell Biology, National Heart and Lung Institute, Bethesda, Maryland, USA

    Edward D. Korn

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© 1977 Plenum Press, New York

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Gaffney, B.J., Chen, SC. (1977). Spin-Label Studies of Membranes. In: Korn, E.D. (eds) Methods in Membrane Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2910-7_4

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  • DOI: https://doi.org/10.1007/978-1-4684-2910-7_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-2912-1

  • Online ISBN: 978-1-4684-2910-7

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