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Part of the book series: Basic Life Sciences ((BLSC,volume 28))

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Abstract

In the past ten years several advances in biotechnology have revolutionized the way fundamental biological problems are approached. I will discuss three of these relatively recently developed disciplines in biotechnology—recombinant DNA, microchemical instrumentation, and monoclonal antibodies—and I will point out the striking synergies that arise from their interactions. These synergies will revolutionize biological research in the near future. I will discuss examples from the health sciences, my area of expertise, yet I suspect many similar applications to your own areas of interest will come to mind. I will review in general terms the basic approaches underlying the recombinant DNA, the microchemical instrumentation, and the monoclonal antibody technologies.

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References

  1. Wood, W.B., J. Wilson, R. Benbow, and L. Hood (1974) Biochemistry: A Problems Approach, 1st Edition. W.A. Benjamin, Inc., Menlo Park, CA (2nd Edition, 1981).

    Google Scholar 

  2. Hunkapiller, M.W., and L.E. Hood (1978) Direct microsequence analysis of polypeptides using an improved sequenator, a nonprotein carrier (Polybrene), and high pressure liquid chromatography. Biochemistry 17:2124–2133.

    Article  PubMed  CAS  Google Scholar 

  3. Henwick, R.M., M.W. Hunkapiller, L.E. Hood, and W.J. Dreyer (1981) J. Biol. Chem. 256:7990–7997.

    Google Scholar 

  4. Hunkapiller, M.W., and L.E. Hood (1983) Protein sequence analysis: Automated microsequencing. Science 219:650–659.

    Article  PubMed  CAS  Google Scholar 

  5. Hunkapiller, M., S. Kent, M. Caruthers, W. Dreyer, J. Firca, C. Giffin, S. Horvath, T. Hunkapiller, P. Tempst, and L. Hood. A microchemical facility for the analysis and synthesis of genes and proteins. Nature (submitted for publication).

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  6. Johnson, N.D., M.W. Hunkapiller, and L.E. Hood (1979) Analysis of phenyl-thiohydantoin amino acids by high performance liquid chromatography on Du Pont Zorbax cyanopropylsilane columns. Anal. Biochem. 100:335–338.

    Article  PubMed  CAS  Google Scholar 

  7. Firca, J., M. Hunkapiller, C. Graham, S. Horvath, M. Caruthers, and L. Hood. An automated DNA synthesizer. (Manuscript in preparation.)

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  8. Bishop, J.M. (1982) Oncogenes. Sci. Amer. 246:80–92.

    Article  PubMed  CAS  Google Scholar 

  9. Doolittle, R.F., M.W. Hunkapiller, L.E. Hood, S.G. Devare, K.C. Robbins, S.A. Aaronson, and H.N. Antoniades (1983) Simian sarcoma virus one gene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor. Science 221: 275–277.

    Article  PubMed  CAS  Google Scholar 

  10. Milstein, C. (1980) Monoclonal antibodies. Sci. Amer. 243: 66–74.

    Article  PubMed  CAS  Google Scholar 

  11. Levy, R., and R.A. Miller (1983) Biological and clinical implications of lymphocyte hybridomas: Tumor therapy with monoclonal antibodies. Ann. Rev. Med. 34:107–116.

    Article  PubMed  CAS  Google Scholar 

  12. Lerner, R.A. (1982) Tapping the immunological repertoire to produce antibodies of predetermined specificity. Nature 299: 592–596.

    Article  CAS  Google Scholar 

  13. Palmiter, R.D., R.L. Brinster, R.E. Hammer, M.E. Trumbauer, M.G. Rosenfeld, N.C. Birnberg, and R.M. Evans (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature 300:611–615.

    Article  PubMed  CAS  Google Scholar 

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© 1984 Plenum Press, New York

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Hood, L.E. (1984). Tools of Genetic Manipulation. In: Omenn, G.S., et al. Genetic Control of Environmental Pollutants. Basic Life Sciences, vol 28. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4715-6_20

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  • DOI: https://doi.org/10.1007/978-1-4684-4715-6_20

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4717-0

  • Online ISBN: 978-1-4684-4715-6

  • eBook Packages: Springer Book Archive

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