Abstract
The base-exchange reaction can form phosphatidylethanolamine (PE) by displacement of a lipid-bound base with free ethanolamine (Porcellati et al., 1971). The reaction requires Ca2+ and it is usually measured incubating free radioactive ethanolamine with membranes in a suitable medium. The PE synthesized in vitro through base-exchange in rat brain microsomes cannot mix readily with the bulk of membrane PE, as shown measuring the reactivity of the lipid with trinitrobenzenesulfonic acid (TNBS) (Corazzi et al., 1983); however, the compartmentation of the newly-formed PE is lost if membranes are mixed with disgregating concentrations of various detergents (Corazzi et al., 1983).
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References
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© 1986 Plenum Press, New York
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Corazzi, L., Arienti, G. (1986). Treatment of Rat Brain Microsomal Vesicles with Octyl-β-D-Glucopyranoside: A Study on Ethanolamine Base-Exchange after Reaggregation. In: Freysz, L., Dreyfus, H., Massarelli, R., Gatt, S. (eds) Enzymes of Lipid Metabolism II. NATO ASI Series, vol 116. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5212-9_11
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DOI: https://doi.org/10.1007/978-1-4684-5212-9_11
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