Abstract
The stimulation of receptors on the cell surface initiates biochemical and physical changes in membranes that lead to biological responses by the cells. The biochemical and physical changes include changes in levels of cyclic nucleotides, phosphorylation of proteins in membranes, increased membrane disorder (fluidity), and increased fluxes of Ca2+, Na+, and other ions. Changes in lipid metabolism associated with receptor stimulation may include the release of arachidonic acid and formation of metabolites and changes in phospholipid N-methylation and in polyphosphoinositide metabolism. The large number of studies on the association of phospholipid N-methylation with receptor stimulation and adenylate cyclase suggest that an important biological mechanism is involved. An overall hypothesis linking receptor stimulation directly with increased activity of AdoMet: PtdEtn methyltransferase and phospholipase A2 was proposed (Hirata and Axelrod, 1980; Mato and Alemany, 1983). This hypothesis is no longer tenable because of difficulties of others with reproduction of the results, problems inherent in the methodology, and errors in the interpretation of the results. Most previous studies failed to recognize that stopping a reaction with acid causes the hydrolysis of plasmalogens to lysoGpl. The presence of lyso compounds was instead interpreted as evidence for phospholipase A2 activity.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Abbreviations
- Gpl:
-
glycerophospholipids
References
Ansell, G.B. and Spanner, S., 1965, The magnesium-ion-dependent cleavage of the vinyl ether linkage of brain ethanolamine plasmalogen. Biochem. J. 94: 252–258.
Arthur, G., Covic,.L., Wientzek, M., and Choy, P.C., 1985, Plasmalogenase in hamster heart. Biochim. Biophys. Acta 833: 189–195.
D’Amato, R.A., Horrocks, L.A. and Richardson, K.E., 1975, Kinetic properties of plasmalogenase from bovine brain. J. Neurochem. 24: 1251–1255.
Freeman, N.M. and Carey, E.M., 1983, Alcohol dehydrogenase-coupled spectrophotometric assay of plasmalogenase. Anal. Biochem. 128: 377–383.
Gunawan, J. and Debuch, H., 1981, Liberation of free aldehyde from 1-(1-alkenyl)-sn-glycero-3-phosphoethanolamine, lysoplasmalogen, by rat liver microsomes. Hoppe-Seylers Z. Physiol. Chem. 362, 445–452.
Gunawan, J. and Debuch, H., 1982, Lysoplasmalogenase - A microsomal enzyme from rat brain. J. Neurochem. 39, 693–699.
Hirata, F. and Axelrod, J., 1980, Phospholipid methylation and biological signal transmission. Science 209: 1082–1090.
Horrocks, L.A. and Fu, S.C., 1978, Pathway for hydrolysis of plasmalogens in brain, in: “Enzymes of Lipid Metabolism”, S. Gatt, L. Freysz and P. Mandel, eds., Plenum Press, New York, Adv. Exptl. Med. Biol. 101: 397–406.
Horrocks, L.A., Yeo, Y.K., Harder, H.W., Mozzi, R., and Goracci, G., 1986, Choline plasmalogens, glycerophospholipid methylation, and receptor-mediated activation of adenylate cyclase. Adv. Cyclic Nucleotide Protein Phosphorylation Res. 20: in press.
Kinlough-Rathbone, J.D., Packham, M.A., and Mustard, J.F., 1983, Measurement of platelet function, in: “Methods in Haematology,” L.A. Harker and T.S. Zimmerman, eds., Churchill-Livingstone, Edinburgh, pp. 64–91.
Mato, J.M. and Alemany, S., 1983, What is the function of phospholipid N-methylation? Biochem. J. 213: 1–10.
Mogelson, S. and Sobel, B.E., 1981, Ethanolamine plasmalogen methylation by rabbit myocardial membranes. Biochim. Biophys. Acta 666:205–211.
Mozzi, R., Siepi, D., Andreoli, V., and Porcellati, G., 1981, The synthesis of choline plasmalogen by the methylation pathway in rat brain. FEBS Lett. 131: 115–118.
Pendley II, C.E. and Horrocks, L.A., 1982, Purification and characterization of beef brain lysophospholipase. Trans. Amer. Soc. Neurochem. 13: 121.
Rittenhouse-Simmons, S., Russell, F.A., and Deykin, D., 1976, Transfer of arachidonic acid to human platelet plasmalogen in response to thrombin. Biochem. Biophys. Res. Commun. 70: 295–301.
Vickers, J.D., Kinlough-Rathbone, R.L., and Mustard, J.F., 1984, Changes in the platelet phosphoinositides during the first minute after stimulation of washed rabbit platelets with thrombin. Biochem. J. 219: 25–31.
Warner, H.R., and Lands, W.E.M., 1961, The metabolism of plasmalogen: Enzymatic hydrolysis of the vinyl ether. J. Biol. Chem. 236: 2404–2409.
Wolf, R.A. and Gross, R.W., 1985, Identification of neutral active phospholipase C which hydrolyzes choline glycerophospholipids and plasmalogen selective phospholipase A2 in canine myocardium. J. Biol. Chem. 260: 7295–7303.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Plenum Press, New York
About this chapter
Cite this chapter
Horrocks, L.A. et al. (1986). Receptor-Mediated Degradation of Choline Plasmalogens and Glycerophospholipid Methylation: A New Hypothesis. In: Freysz, L., Dreyfus, H., Massarelli, R., Gatt, S. (eds) Enzymes of Lipid Metabolism II. NATO ASI Series, vol 116. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5212-9_85
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5212-9_85
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5214-3
Online ISBN: 978-1-4684-5212-9
eBook Packages: Springer Book Archive