Abstract
Granzyme A, initially termed Hanukkah factor (HF) in this laboratory, is a serine protease contained within specialized cytoplasmic granules of activated cytolytic lymphocytes (CTL). These granules contain other granzymes, proteoglycans, and a pore-forming protein termed perforin (Podack, 1989). The granules and the proteins contained within them appear only after T-cell activation occurs, and their exocytosis seems to account for at least one mechanism of lymphocyte cytotoxicity. Whether this is the only mechanism remains controversial (Chapters 16, 18, 28, this volume; Berke, 1991; Krahenbuhl and Tschopp, 1991).
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References
Berke G (1991): Lymphocyte-triggered internal target disintegration. Immunology Today 12: 396–399
Chen RH, Ivens KW, et al. (1993): The use of granzyme A as a marker of heart transplant rejection in cyclosporine or anti-CD4 monoclonal antibody-treated rats transplantation (in press)
Garcia SJ, Macdonald HR, et al. (1990): Cell specificity of granzyme gene expression. J Immunol 145: 3111–3118
Garcia SJ, Velotti F, et al. (1988): Appearance of granule-associated molecules during activation of cytolytic T-lymphocyte percursors by defined stimuli. Immunology 64: 129–134
Gershenfeld HK, Hershberger KJ, et al. (1988): Cloning and chromosomal assignment of a human cDNA encoding a T cell-and natural killer cell-specific trypsinlike protease. Proc Natl Acad Sci USA 85: 1184–1188
Gershenfeld NK, Weissman IL (1986): Cloning of a cDNA for a T cell-specific serine protease from a cytotoxic T lymphocyte. Science 232: 854–858
Griffiths GM, Alpert S, et al. (1992): Perforin and granzyme A expression identifying cytolytic lymphocytes in rheumatoid arthritis. Proc Natl Acad Sci USA 89: 549–553
Griffiths GM, Mueller C (1991): Expression of perforin and granzymes in vivo: Potential diagnostic markers for activated cytotoxic cells. Immunol Today 12: 415–419
Griffiths GM, Namikawa R, et al. (1991): Granzyme A and perforin as markers for rejection in cardiac transplantation. Eur J Immunol 21: 687–692
Hayes, MP, Berrebi GA, et al. (1989): Induction of target cell DNA release by the cytotoxic T lymphocyte granule protease granzyme A. J Exp Med 170: 933–946
Held W, MacDonald HR, et al. (1990): Genes encoding tumor necrosis factor alpha and granzyme A are expressed during development of autoimmune diabetes. Proc Natl Acad Sci USA 87: 2239–2243
Hershberger RJ, Gershenfeld HG, et al. (1992): Genomic organization of the mouse granzyme A gene. J Biol Chem (in press)
Krahenbuhl O, Tschopp J (1991): Immunol Today 12: 399–402
Kramer MD, Fruth U, et al. (1989): Expression of cytoplasmic granules with T cell associated serine protease 1 activity in Ly2 (CD8+) T lymphocytes responding to lymphocytic choriomeningitis virus in vivo. Eur J Immunol 19: 151–156
Masson D, Tschopp J (1985): Isolation of a lytic, por-forming protein (perforin) from cytolytic T-lymphocytes. J Biol Chem 260: 9069–9072
McWhinnie DL, Carter NP, et al. (1985): Is the T4/T8 ratio an irrelevance in renal transplantation monitoring? Transplant Proc 17: 2548–2549
Mueller C, Gershenfeld HG, et al. (1988): A high proportion of T lymphocytes that infiltrate H-2 incompatible heart allografts in vivo express genes encoding cytotoxic cell specific serine proteases, but do not express the MEL-14 defined homing receptor. J Exp Med 167: 1124–1136
Muller C, Kagi D, et al. (1989): Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus. Eur J Immunol 19: 1253–1259
Podack ER (1989): Killer lymphocytes and how they kill. Curr Opinions Cell Biol 1: 929–933
Velotti F, Palmieri G, et al. (1989): Granzyme A expression by normal rat natural killer (NK) cells in vivo and by interleukin 2-activated NK cells in vitro. Eur J Immunol 19: 575–578
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© 1993 Birkhäuser Boston
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Griffiths, G.M., Alpert, S., Hershberger, R.J., Su, L., Weissman, I.L. (1993). The Granzyme A Gene: A Marker for Cytolytic Lymphocytes In Vivo. In: Sitkovsky, M.V., Henkart, P.A. (eds) Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods. Birkhäuser Boston. https://doi.org/10.1007/978-1-4684-6814-4_25
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DOI: https://doi.org/10.1007/978-1-4684-6814-4_25
Publisher Name: Birkhäuser Boston
Print ISBN: 978-1-4684-6816-8
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