Abstract
Iodine-125, when incorporated into the DNA of microorganisms as iododeoxyuridine, has been demonstrated to induce double-strand DNA breaks (DSBs) with approximately 100% efficiency when it undergoes radioactive decay. In the biological dose range approximately 2/3 of these breaks are repaired in a wild type (rec+) strain of E. coli K12, but none are repaired in the corresponding recombination deficient (recA) strain. Each unrepaired DSB appears to produce a lethal event in either strain. Survival experiments imply that maximum repair of DSBs in rec+ cells can occur in the presence of only a single genome per cell and therefore does not require a recombi-national event. However, experiments with 125I-labelled small intracellular closed circular DNA mulecules (super-infecting phage λ, MW=33×106d) demonstrate no repair of decay-induced DSBs, suggesting that the superstructure of the large cellular chromosome plays an important role in the repair of DSBs, even in the absence of recombination.
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© 1981 Plenum Press, New York
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Krisch, R.E. (1981). 125I Decay in Microorganisms: A Prokaryotic Model System for Examining the Role of Unrepaired Double-Strand DNA Breaks in Cell Killing. In: Seeberg, E., Kleppe, K. (eds) Chromosome Damage and Repair. NATO Advanced Study Institutes Series, vol 40. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-7956-0_25
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DOI: https://doi.org/10.1007/978-1-4684-7956-0_25
Publisher Name: Springer, New York, NY
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