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Labeling with Gaseous 14CO2 or by Feeding on Radioactive Tissues

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Hydra: Research Methods
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Abstract

To label hydra directly by feeding them on tissues that have been previously made radioactive or by exposing them to 14CO2, The ingested radioactive tissue labels the endoderm first and, within a day, most of the hydra’s cells (Lenhoff, 1961). The labeled 14CO2 passes directly into hydra and labels most intensely the interstitial cells and their derivative cells (Lenhoff, 1959); in the case of green hydra, the 14CO2 labels the endosymbiotic algae most intensely (Muscatine and Lenhoff, 1965).

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References

  • Cowie, D. B., Bolton, E. T., and Sands, M. K. 1952. The labeling of bacterial cells with S35 For the production of high specific activity compounds. Arch. Biochem. Biophys. 35:140–145

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  • Lenhoff, H. M. 1959. Migration of 14C-labeled cnidoblasts. Exp. Cell Res. 17: 570–573.

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  • Lenhoff, H. M. 1961. Digestion of protein in hydra as studied using radioautography and fractionation by differential solubilities. Exp. Cell Res. 23:335–353

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  • Muscatine, L., and Lenhoff, H. M. 1963. Symbiosis: On the role of algae symbiotic with hydra. Science 142: 956–958.

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© 1983 Springer Science+Business Media New York

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Lenhoff, H.M. (1983). Labeling with Gaseous 14CO2 or by Feeding on Radioactive Tissues. In: Lenhoff, H.M. (eds) Hydra: Research Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0596-6_27

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  • DOI: https://doi.org/10.1007/978-1-4757-0596-6_27

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-0598-0

  • Online ISBN: 978-1-4757-0596-6

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