Abstract
A selective and sensitive HPLC method has been developed for the determination of glucuronides in plasma and urine. Prior to chromatography the carboxylic acid function of the glucuronic acid moiety is converted into a fluorescent amide by a method selective for the carboxyl group. The acidic function is first activated with 2-bromo-1-methylpyridinium iodide and then reacted with the fluorescence label [N-(1-dim ethyl aminonaphthalenesulphonyl)cadaverine or N-(1-naph-thyl)ethylenediamine] to yield the amide. The derivatization products are separated by a two-column system in combination with a column-switching technique. For α-naphthyl-β-D-glucuronide, β-oestradiol-17-β-D-glucuronide and some other glucuronides, consideration is given to chromatographic properties, intrinsic fluorescence sensitivities (both of reagents and of derivatives) and clean-up procedures.
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Lingeman, H., Meussen, G.W.M., van der Zouwen, C., Underberg, W.J.M., Hulshoff, A. (1986). Pre-Column (HPLC) Fluorescence Labelling of Glucuronides. In: Reid, E., Scales, B., Wilson, I.D. (eds) BIOACTIVE ANALYTES, Including CNS Drugs, Peptides, and Enantiomers. Methodological Surveys in Biochemistry and Analysis, vol 16 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1892-8_24
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