Abstract
Three-dimensional (3D) microscopy requires the collection of data over a certain volume in the specimen, followed by a suitable two-dimensional (2D) visualization procedure in which the desired image is produced. In holography, nature offers us a potential pathway by which the 3D structure of an object can be observed directly through wavefront reconstruction techniques. However, the slow time response of holographic recording media prevents real-time applications of this technique. In addition, while in principle this approach might work in reflection, holographic fluorescence images are impossible because emitted fluorescence radiation is incoherent.
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Brakenhoff, G.J., Visscher, K. (1995). Real-Time Stereo (3D) Confocal Microscopy. In: Pawley, J.B. (eds) Handbook of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5348-6_21
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DOI: https://doi.org/10.1007/978-1-4757-5348-6_21
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