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RNA Binding by Members of the 70-kDa Family of Molecular Chaperones

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RNA Binding Proteins

Part of the book series: Endocrine Updates ((ENDO,volume 16))

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Abstract

Research on heat shock and other stress proteins (hsps) has revealed a number of intriguing aspects about this remarkable class of molecules. First, hsps are highly abundant proteins in cells even under non-stress conditions. This abundance holds for virtually all organisms or cell types examined. Second, hsps are the most phylogenetically conserved proteins known to biology with an overall primary amino acid sequence homology of some 50 % between Escherichia coli and man. Third, hsps have been implicated in a myriad of cellular processes throughout the years. Although the majority of these biological functions delineate hsps as molecular chaperones, recent evidence suggests that certain heat shock proteins possess a likely ancient, evolutionarily conserved role pointing beyond their classical chaperoning function. This chapter deals with a recently described novel feature of the mammalian 70-kDa super-family of molecular chaperones (hsp70, hsc70, hsp110 and grp170), their inherent RATA binding properties. The monograph highlights the RATA sequence preference as well as the RNA-binding domain of these proteins. The influence of ATP and a peptide substrate on RNA-binding —all key components in chaperoning function- will also be detailed. Although the data were obtained using both hsp/hsc70 and hsp110 as the RATA binding partner, since most of the supporting evidence deals with hsp70/hsc70, discussions of possible in vivo functions will mainly be extended to these widely characterized stress proteins.

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Authors and Affiliations

  1. INTERCELL, Vienna, Austria

    Christine Zimmer, Eszter Nagy & Tamás Henics

  2. Roswell Park Cancer Inst., Buffalo, NY, USA

    John Subjeck

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  1. Christine Zimmer
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  2. Eszter Nagy
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Editors and Affiliations

  1. Departments of Medicine and Physiology & Biophysics, Georgetown University School of Medicine, Washington, DC, USA

    Kathryn Sandberg Ph.D. (Associate Professor) (Associate Professor)

  2. Department of Physiology & Biophysics, Georgetown University School of Medicine, Washington, DC, USA

    Susan E. Mulroney Ph.D. (Associate Professor) (Associate Professor)

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Zimmer, C., Nagy, E., Subjeck, J., Henics, T. (2002). RNA Binding by Members of the 70-kDa Family of Molecular Chaperones. In: Sandberg, K., Mulroney, S.E. (eds) RNA Binding Proteins. Endocrine Updates, vol 16. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6446-8_7

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  • DOI: https://doi.org/10.1007/978-1-4757-6446-8_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4419-4935-6

  • Online ISBN: 978-1-4757-6446-8

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