Abstract
High-resolution 1H NMR spectroscopy is valuable in the bioanalysis of biological fluids (e.g. [1–6], and Vols. 16 & 17 in this series — J.K. Nicholson). Notably, it allows quantitative information on a wide range of endogenous and xenobiotic metabolites to be collected simultaneously. As NMR is non-selective in the detection of metabolites (all compounds present at >0.1 mM levels being detectable), it is particularly appropriate for the investigation of the metabolism of compounds with poorly understood metabolic profiles.
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Abbreviations
- COSY:
-
1H-shift correlated
- Fid:
-
free induction decay [cf. GC use of ‘FID’-Ed,]
- HOPC:
-
1,4,5,6-tetrahydro-6-oxo-3-pyridazine carboxylic acid
- TCA:
-
trichloroacetic acid
- TSP:
-
sodium d4-(trimethylsilyl)propionate
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© 1988 Springer Science+Business Media New York
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Sanins, S.M., Timbrell, J.A., Elcombe, C., Nicholson, J.K. (1988). Proton NMR Studies on the Metabolism and Biochemical Effects of Hydrazine In Vivo . In: Reid, E., Robinson, J.D., Wilson, I.D. (eds) Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular. Methodological Surveys in Biochemistry and Analysis, vol 18 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9424-3_53
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DOI: https://doi.org/10.1007/978-1-4757-9424-3_53
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