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Mechanisms of Injury and Protection in Living Cells and Tissues at Low Temperatures

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Current Trends in Cryobiology

Part of the book series: The International Cryogenics Monograph Series ((INCMS))

Abstract

There is an apparent contradiction between the use of very low temperatures to store isolated cells and tissues for long periods and the experimental finding that living cells are usually destroyed by freezing. It is in understanding ways of overcoming the damage caused by freezing that progress towards the very long-term storage of cells has been achieved.

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References

  1. Gehenio, P. M., Rapatz, G. L., and Luyet, B., “Effects of freezing velocities in causing or preventing haemolysis,” Biodynamica, 9, 77–82 (1963).

    Google Scholar 

  2. Levitt, J., “A sulfhydryl-disulfide hypothesis of frost injury and resistance in plants,” J. Theoret. Biol., 3, 355–391 (1962).

    Article  Google Scholar 

  3. Lovelock, J. E., “The haemolysis of human red blood-cells by freezing and thawing,” Biochim. Biophys. Acta, 10, 414–426 (1953).

    Article  Google Scholar 

  4. Lovelock, J. E., “The mechanism of the protective action of glycerol against haemolysis by freezing and thawing,” Biochim. Biophys. Acta, 11, 28–36 (1953).

    Article  Google Scholar 

  5. Mazur, P., “Kinetics of water loss from cells at subzero temperatures and the likelihood of intracellular freezing,” J. Gen. Physiol., 47, 347–369 (1963).

    Article  Google Scholar 

  6. Stephenson, J. L., “Fundamental physical problems in the freezing and drying of biological materials,” in (Parkes, A. S. and Smith, A. U., eds.) Recent Research in Freezing and Drying, pp. 121–145, Blackwell, Oxford (1960).

    Google Scholar 

  7. Polge, C., Smith, A. U., and Parkes, A. S., “Revival of spermatozoa after vitrification and dehydration at low temperatures,” Nature (London), 165, 666 (1949).

    Article  Google Scholar 

  8. Farrant, J., “The preservation of living cells, tissues, and organs at low temperatures: some underlying principles,” Lab. Pract., 15, 402–404 (1966).

    Google Scholar 

  9. Miner, C. S. and Dalton, N. N. (eds.) Glycerol, ACS #117, Reinhold Book Corporation, New York (1953).

    Google Scholar 

  10. Huggins, C. E., “Prevention of haemolysis of large volumes of red blood cells slowly frozen and thawed in the presence of dimethyl sulphoxide,” Transfusion (Philadelphia) 3, 483–493 (1963).

    Article  Google Scholar 

  11. Farrant, J., “Mechanisms of cell damage during freezing and thawing and its prevention,” Nature (London) 205, 1284–1287 (1965).

    Article  Google Scholar 

  12. Rapatz, G. and Luyet, B., “Effects of cooling rates on the preservation of erythrocytes in frozen blood containing various protective agents,” Biodynamica, 9, 333–350 (1965).

    Google Scholar 

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© 1970 Springer Science+Business Media New York

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Farrant, J. (1970). Mechanisms of Injury and Protection in Living Cells and Tissues at Low Temperatures. In: Smith, A.U. (eds) Current Trends in Cryobiology. The International Cryogenics Monograph Series. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9933-0_5

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  • DOI: https://doi.org/10.1007/978-1-4757-9933-0_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-9935-4

  • Online ISBN: 978-1-4757-9933-0

  • eBook Packages: Springer Book Archive

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