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Intracellular Trafficking of Sterols

  • Chapter
Subcellular Biochemistry

Part of the book series: Subcellular Biochemistry ((SCBI,volume 16))

Abstract

Sterols are almost universally found as major membrane components of eukaryotic cells but are absent among prokaryotes. Whereas prokaryotic cells contain only a plasma membrane, eukaryotes have evolved an elaborate network of membrane-bounded organelles, or “membranelles,” in addition to the plasma membrane. Interestingly, the majority of cellular free cholesterol resides in the plasma membrane, with cholesterol-to-phospholipid (C/P) ratios several-fold higher than those of intracellular membranes. Thus, the lipid aspect of intracellular membranes of eukaryotes biochemically resembles the prokaryotic plasma membrane.

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Abbreviations

ACAT:

acyl-CoA-cholesterol acyltransferase

AMG:

ami-noglutethimide

CHO:

Chinese hamster ovary

CBP:

cholesterol-binding protein

CoA:

coenzyme A

C/P:

cholesterol-to-phospholipid ratio

DEAE:

diethylaminoethyl

EM:

electron microscope

ER:

endoplasmic reticulum

HDL:

high-density lipoprotein

HMGR:

2-hydroxy-2-methyl-glutaryl-CoA reductase

LDL:

low-density lipoprotein

RER:

rough endoplasmic reticulum

S105 :

105,000 × g supernatant

SCP:

sterol carrier proteins

SCP1 :

sterol carrier protein 1

SCP2 :

sterol carrier protein 2

SER:

smooth endoplasmic reticulum

SPF:

supematant protein factor.

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Authors and Affiliations

  1. Medical Products Department, Experimental Station, E. I. du Pont de Nemours & Co., Wilmington, Delaware, 19880-0400, USA

    J. T. Billheimer

  2. USDA-ARS-ERRC, Wyndmoor, Pennsylvania, 19118, USA

    M. P. Reinhart

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  1. J. T. Billheimer
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  1. RUCA-Laboratory for Human Biochemistry, University of Antwerp, B2020, Antwerp, Belgium

    H. J. Hilderson

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Billheimer, J.T., Reinhart, M.P. (1990). Intracellular Trafficking of Sterols. In: Hilderson, H.J. (eds) Subcellular Biochemistry. Subcellular Biochemistry, vol 16. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1621-1_10

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