Abstract
The development of prostate cancer has been linked to high level of dietary fat intake. Our laboratory investigates the connection between cancer cell growth and fatty acid products. Studying human prostatic carcinoma PC-3 cells, we found that prostaglandin E2 (PGE2) increased cell growth and up-regulated the gene expression of its own synthesizing enzyme, cyclooxygenase-2 (COX-2). PGE2 increased COX-2 mRNA expression dose-de-pendently with the highest levels of stimulation seen at the 3-hour period following PGE2 addition. The NSAID flurbiprofen (5 uM), in the presence of exogenous PGE2, inhibited the up-regulation of COX-2 mRNA and cell growth. These data suggest that the levels of local intracellular PGE2 play a major role in the growth of prostate cancer cells through an activation of COX-2 gene expression.
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Bibliography
W. L. Xie, J. G. Chipman, D. L. Robertson, R. L. Erikson, D. L. Simmons, Expression of mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing, Proc. Natl. Acad. Sci. U.S.A. 88:2692 (1991).
H. R. Herschman, B. S. Fletcher, D. A. Kujubu, TIS 10, a mitogen-inducible glucocorticoid-inhibited gene that encodes a second prostaglandin synthase/cyclooxygenase enzyme, J. Lipid Medial 6:89 (1993).
A. Ristimaki, S. Garfinkel, J. Wessendorf, T. Maciag, T. Hla, Induction of cyclooxygenase-2 by interleukin-1 alpha: evidence for post-transcriptional regulation, J. Biol. Chem. 269:11769 (1994).
S. B. Appleby, A. Ristimaki, K. Neilson, K. Narko, T. Hla, Structure of the human cyclo-oxygenase-2 gene, Biochem. J. 302:723 (1994).
D. P. Rose, J. M. Connolly, Effects of fatty acids and eicosanoid synthesis inhibitors on the growth of two human prostate cancer cell lines, Prostate 18:243 (1991).
J. M. Connolly, D. P. Rose, Interactions between epidermal growth factor-mediated autocrine regulation and linoleic acid-stimulated growth of a human prostate cancer cell line, Prostate 20:151 (1992).
D. P. Rose, J. M. Connolly, Dietary fat, fatty acids and prostate cancer, Lipids 27:798 (1992).
A. Chaudry, S. McClinton, L. E. Moffat, K. W. Wahle, Essential fatty acid distribution in the plasma and tissue phospholipids of patients with benign and malignant prostatic disease, Br. J. Cancer 64:1157 (1991).
A. A. Chaudry, K. W. Wahle, S. McClinton, L. E. Moffat, Arachidonic acid metabolism in benign and malignant prostatic tissues in vitro: effects of fatty acids and cyclooxygenase inhibitors, Int. J. Cancer 57:176 (1994).
M. Hughes-Fulford, N. H. Bisphoric, Up-regulation of c-fos, c-jun and gamma-and beta-actin messages by prostaglandin E2, submitted for publication.
M. Hughes-Fulford, R. Appel, M. Kumegawa, J. Schmidt, Effect of dexamethasone on proliferating osteoblasts: inhibition of prostaglandin E2 synthesis, DNA synthesis, and alterations in actin cytoskeleton, Exp. Cell. Res. 203:150 (1992).
P. Garrone, L. Galibert, F. Rousset, S. M. Fu, J. Banchereau, Regulatory effects of prostaglandin E2 on the growth and differentiation of human B lymphocytes activated through their CD41 antigen, J. Immunol. 152:4282 (1994).
M. J. Thun, M. M. Namboodiri, C. Heath, Jr., Aspirin use and reduced risk of fatal colon cancer, N. Engl. J. Med. 325:1593 (1991).
D. L. Earnest, L. J. Hixson, D. S. Alberts, Piroxicam and other cyclooxygenase inhibitors: potential for cancer chemoprevention, J. Cell. Biochem. Suppl. 156(1992).
D. A. Kujubu, B. S. Fletcher, B. C. Varnum, R. W. Lim, H. R. Herschman, TIS 10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue, J. Biol. Chem. 266:12866 (1991).
U. Danesch, P. C. Weber, A. Sellmayer, Arachidonic acid increases c-fos and Erg-1 mRNA in 3T3 fibroblasts by formation of prostaglandin E2 and activation of protein kinase C., J. Biol. Chem. 269:27258 (1994).
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Tjandrawinata, R.R., Hughes-Fulford, M. (1997). Up-Regulation of Cyclooxygenase-2 by Product-Prostaglandin E2 . In: Honn, K.V., Marnett, L.J., Nigam, S., Jones, R.L., Wong, P.YK. (eds) Eicosanoids and other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury 3. Advances in Experimental Medicine and Biology, vol 407. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1813-0_25
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DOI: https://doi.org/10.1007/978-1-4899-1813-0_25
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