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Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder Layer-Free Conditions

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Atlas of Human Pluripotent Stem Cells

Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

While the culturing of human embryonic stem cells (hESCs) and induced pluripotent cells (iPSCs) with mouse embryonic fibroblasts (MEFs) or human foreskin fibroblasts may be adequate for research purposes, it is less suitable for clinical and industrial use due to the risk of exposure of the cells to animal pathogens and to variations between batches of MEFs and fetal bovine serum (FBS). To establish reproducible and defined cultures, devoid of animal products, feeder layers and ­animal products need to be replaced. This chapter describes layer-free culture ­systems that have been developed specifically for iPSCs.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology Rambam Health Care Campus Stem Cell Research Center Rapapport Faculty of Medicine, Technion – Israel Insitute of Technology, Haifa, Israel

    Michal Amit PhD & Joseph Itskovitz-Eldor MD DSc

Authors
  1. Michal Amit PhD
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  2. Joseph Itskovitz-Eldor MD DSc
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Corresponding author

Correspondence to Michal Amit PhD .

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Editors and Affiliations

  1. Rapapport Faculty of Medicine, Obstetrics and Gynecology, Technion–Israel Institute of Technology, Haifa, 31 096, Israel

    Michal Amit

  2. Rapapport Faculty of Medicine, Obstetrics and Gynecology, Technion-Israel Institute of Technology, Haifa, 31 096, Israel

    Joseph Itskovitz-Eldor

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Amit, M., Itskovitz-Eldor, J. (2012). Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder Layer-Free Conditions. In: Amit, M., Itskovitz-Eldor, J. (eds) Atlas of Human Pluripotent Stem Cells. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-548-0_3

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