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Sperm DNA Fragmentation in Human Split Ejaculates

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  • First Online:
XIIIth International Symposium on Spermatology

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Abstract

Background: Standard semen evaluation according to WHO thresholds (2010) is still insufficient to disclose individuals with a diagnosis of idiopathic infertility. Sperm DNA fragmentation is considered a possible valuable marker, and flow-cytometry-based Sperm Chromatin Structure Assay (SCSA) analysis has been used. However, the method is cumbersome and time-consuming; hardly applicable in a clinical setting where split ejaculates are used for diagnostics and intervention. The present study explores an alternative method using conventional bright-field microscopy (HalospermG2, Halothech, Madrid, Spain) with Sperm DNA fragmentation (SDF) as end values. Analyses were done on split ejaculates (F1, sperm-rich fraction, mainly prostate-dominated versus F2, sperm-poor fraction, mainly seminal vesicle-dominated) of documented fertile donors and partners in a couple seeking for ART-intervention with a clinical diagnosis of idiopathic infertility (patients).

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Reference

  • Alvarez-Rodriguez M, Liffner S, Vicente-Carrillo A, Hammar M, Rodriguez-Martinez H (2016) Sperm chromatin integrity (SCSA analysis) in specific ejaculate fractions can improve semen evaluation under clinical settings. Annual meeting ESHRE, Helsinki, Finland, 2016. Human Reproduction 31 S1 i163-i164

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Authors and Affiliations

  1. Department of Biomedical and Clinical Sciences BKV, BKH, Obst&Gyn, Faculty of Medicine and Health Sciences, Linköping University, Linköping, Sweden

    M. Alvarez-Rodríguez, I. Pehrson, S. Liffner, M. Hammar & H. Rodríguez-Martinez

Authors
  1. M. Alvarez-Rodríguez
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  2. I. Pehrson
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  3. S. Liffner
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  4. M. Hammar
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  5. H. Rodríguez-Martinez
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Corresponding author

Correspondence to M. Alvarez-Rodríguez .

Editor information

Editors and Affiliations

  1. Andrology Laboratory, ANOVA Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden

    Lars Björndahl

  2. Andrology Laboratory, ANOVA Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden

    John Flanagan

  3. Andrology Laboratory, ANOVA Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden

    Rebecka Holmberg

  4. Andrology Laboratory, ANOVA Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden

    Ulrik Kvist

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Alvarez-Rodríguez, M., Pehrson, I., Liffner, S., Hammar, M., Rodríguez-Martinez, H. (2021). Sperm DNA Fragmentation in Human Split Ejaculates. In: Björndahl, L., Flanagan, J., Holmberg, R., Kvist, U. (eds) XIIIth International Symposium on Spermatology. Springer, Cham. https://doi.org/10.1007/978-3-030-66292-9_12

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