Abstract
The results of successful long-term preservation by various methods (cryopreservation, lyophilization, preservation on anhydrous silica gel and in sterile soil) are presents for different taxonomic groups of fungi preserved in All-Russian Collection of Microorganisms (VKM), G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS). For each species, data are given on the number of strains studied and the maximum time of maintaining vitality. The protocol of method “drying on anhydrous silica gel” with the following storage at different temperatures is given.
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1.1.1 Annex 1: Fields Attributes in the Table «Database Preservation Methods»
Code | Counter |
EntryDate | Date/Time |
Method | Text |
Col | Text |
Strain | Numerical |
dep | Text |
pat | Text |
Curator | Text |
Dubl-cart | Text |
Dubl fond | Text |
Dubl fond new | Text |
Ampules | Numerical |
Data | Date/Time |
Result | Text |
Data2 | Date/Time |
Result2 | Text |
Days | Numerical |
Year | Numerical |
Comments | Text |
Data3 | Date/Time |
Result3 | Text |
Data4 | Date/Time |
Result4 | Text |
Data5 | Date/Time |
Result5 | Text |
EditDate | Date/Time |
Protector | Text |
Programm | Text |
Location | Text |
Type | Text |
1.1.2 Annex 2: Maximal Preservation Times for VKM Fungal Species
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Ozerskaya, S.M., Ivanushkina, N.E., Kochkina, G.A., Danilogorskaya, A.A., Pinchuk, I.P., Vasilenko, A.N. (2022). Various Methods of Long-Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM). In: Gupta, V.K., Tuohy, M. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-030-83749-5_1
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DOI: https://doi.org/10.1007/978-3-030-83749-5_1
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