Abstract
The ocean is a salty and very diluted broth of organic matter that contains about 680 Pg C. More than 97% of this organic matter is in the dissolved form. This huge amount of reduced carbon, comparable to the CO2 accumulated in the atmosphere, results in an average concentration of 0.48 ppm (or 40.3 μmol kg−1) when divided by the world ocean volume. Comparatively, salts add up to about 35,000 ppm. Low concentrations, extreme complexity of an organic matrix formed by myriads of compounds and vast interferences with the salt matrix are the main challenges associated with the accurate determination of dissolved organic matter (DOM) in the marine environment. In this chapter we aim to provide a useful overview of the procedures to effectively sample (“clean lab” protocols), process (filtration, ultrafiltration, solid-phase extraction), preserve (acidification, freezing, freeze-drying) and analyse the bulk DOM pool. Analytical methods for the characterization of its elemental (C, N, P), optical (absorption and fluorescence spectroscopy) and emerging molecular property (FT-ICR-MS, NMR) composition will be described. Out of the scope of this chapter is the determination of individual compounds (e.g. amino acids, carbohydrates) as well as the isotope characterization of DOM.
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Acknowledgements
We wish to express our gratitude to Dr. Sahithya Phani Babu Vemulapalli and Prof. Thorsten Dittmar for their critical reading of the chapter and their useful suggestions. XAA-S and MN-C acknowledge financial support from the Spanish “Plan Estatal” project e-IMPACT (grant number PID2019-109084RB-C22). PER acknowledges financial support from the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (ERC Synergy Grant “Deep Purple” under grant agreement No. 856416). PER also thanks the funding from Schlumberger oil service and ERC project ABYSS (Grant N° 294757 to Antje Boetius) for their support during the 10 years of work on FT-ICR-MS at the laboratory of Thorsten Dittmar. MJ Pazó helped with the preparation of some figures.
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Álvarez-Salgado, X.A., Nieto-Cid, M., Rossel, P.E. (2023). Dissolved Organic Matter. In: Blasco, J., Tovar-Sánchez, A. (eds) Marine Analytical Chemistry. Springer, Cham. https://doi.org/10.1007/978-3-031-14486-8_2
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