Summary
Mapping of complex genomes has been influenced substantially by the isolation of locus-specific, but repetitive DNA elements known as VNTRs. Since a high GC-content is characteristic of most of these elements one might expect them to be clustered at least partially in CpG-islands. To address this question we have constructed a porcine liver DNA NotI-linkage library in pUC18 using isolated HTF-islands. HpaII tiny fragments ranging from less than 100 bp to 1 kb in length were randomly selected and analysed by sequencing. As expected from the source of DNA the clones were rich in CpG-, C- and G-content. Several clones exhibiting a repetitive (GGC)n, motif and sequences originally present in human VNTR-markers were shown to detect DNA fingerprints in different species (Homo sapiens, Sus scrofa domestica,Gallus domesticus, Glycine max, Saccharomyces cerevisiae). A subset of clones was used for hybridization experiments with porcine DNA and were shown to detect single-copy linkage fragments.
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© 1991 Birkhäuser Verlag Basel/Switzerland
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Brenig, B., Brem, G. (1991). Human VNTR Sequences in Porcine HTF-Islands. In: Burke, T., Dolf, G., Jeffreys, A.J., Wolff, R. (eds) DNA Fingerprinting: Approaches and Applications. Experientia Supplementum, vol 58. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7312-3_3
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DOI: https://doi.org/10.1007/978-3-0348-7312-3_3
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