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Phosphoinositidase C, Inositol Polyphosphates and Force Generation of Airways Smooth Muscle

  • Chapter
Airways Smooth Muscle: Biochemical Control of Contraction and Relaxation

Part of the book series: Respiratory Pharmacology and Pharmacotherapy ((RPP))

  • 62 Accesses

Abstract

Agonist-stimulated hydrolysis of phosphatidylinositol-4,5-bisphosphate (PtdIns(4, 5) P2) is now thought to be one of the major transmembrane signalling pathways involved in pharmacomechanical coupling in airways smooth muscle (ASM) [1–3]. The enzyme which plays such a pivotal role in this process, namely phosphoinositidase C (PIC), is a phosphodiesterase of the phospholipase C type and is responsible for the cleavage of PtdIns(4, 5) P2 into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) which serve to activate protein kinase C (PKC) and release intracellular Ca2+ respectively. The early studies which began to implicate a second-messenger function for Ins(1,4, 5) P3 in ASM contraction [4–7] have fuelled much research in this area not least because they offered one of the first plausible mechanisms whereby receptor activation could be linked to the release of intracellular Ca2+ in a manner that was not dependent on changes in membrane potential. It is now clear however, that a number of other membrane associated ‘phosphodiesterase-like’ enzymes including phospholipase D and phospholipase A2 may also be involved in integrating the response of ASM cells to incoming hormonal or neurotransmitter signals.

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  1. Department of Medicine (RIE), Rayne Laboratory, City Hospital, Respiratory Medicine Unit, Edinburgh, United Kingdom

    Edwin R. Chilvers

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  1. Department Head Discovery Biology, Rhône-Poulenc Rorer Ltd, Dagenham Research Centre, RM10 7XS, Dagenham, Essex, England

    David Raeburn

  2. Department of Thoracic Medicine, Royal Brompton National Heart and Lung Institute, Dovehouse Street, SW3 6LY, London, England

    Mark A. Giembycz (Lecturer) (Lecturer)

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© 1994 Springer Basel AG

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Chilvers, E.R. (1994). Phosphoinositidase C, Inositol Polyphosphates and Force Generation of Airways Smooth Muscle. In: Raeburn, D., Giembycz, M.A. (eds) Airways Smooth Muscle: Biochemical Control of Contraction and Relaxation. Respiratory Pharmacology and Pharmacotherapy. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-7681-0_4

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  • DOI: https://doi.org/10.1007/978-3-0348-7681-0_4

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-7683-4

  • Online ISBN: 978-3-0348-7681-0

  • eBook Packages: Springer Book Archive

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