Abstract
Different experimental techniques are available to study the binding of RNA-binding proteins (RBPs) to RNA at high resolution with high-throughput methods. In this chapter, the underlying approaches of crosslinking and immunprecipitation (CLIP) of RBP-bound RNA fragments are presented. The computational analysis and filtering of the resulting sequencing data are explained. The design and function of the computational PAR-CLIP analysis pipeline used in the Rajewsky lab is presented, with a special emphasis on controlling false-positive read mapping errors.
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Jens, M. (2014). Computational Analysis of PAR-CLIP Data. In: Dissecting Regulatory Interactions of RNA and Protein. Springer Theses. Springer, Cham. https://doi.org/10.1007/978-3-319-07082-7_2
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DOI: https://doi.org/10.1007/978-3-319-07082-7_2
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