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Mechanisms of Signal Transduction

  • Chapter
Introduction to Fluorescence Sensing

Abstract

In previous chapters we discussed two essential functional components of every fluorescence sensor – the binding-recognition units (binders or receptors) and the units providing the response signal (reporters). Here we focus on the coupling between these functionalities. Such coupling must involve signal transduction resulting in changes on target biding of the reporter emission. The coupling mechanisms can be based on the excited-state reactions, such as electron transfer (intramolecular or intermolecular), intramolecular charge transfer, proton transfer or excited-state energy transfer. Conformational changes in receptor unit coupled with target binding may generate the reporter signal if they influence these reactions. In the case of several binding sites coupled with response functionality, nonlinear response functions can be obtained and simple logical operations realized.

How to couple the recognition and reporting functionalities? This is the theme of general discussion at the end of this Chapter with questions and problems addressed to the reader.

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  1. Laboratory of Nanobiotechnology Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine

    Alexander P. Demchenko

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Demchenko, A.P. (2015). Mechanisms of Signal Transduction. In: Introduction to Fluorescence Sensing. Springer, Cham. https://doi.org/10.1007/978-3-319-20780-3_8

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