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Controlling the Editor: The Many Roles of RNA-Binding Proteins in Regulating A-to-I RNA Editing

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RNA Processing

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 907))

Abstract

RNA editing is a cellular process used to expand and diversify the RNA transcripts produced from a generally immutable genome. In animals, the most prevalent type of RNA editing is adenosine (A) to inosine (I) deamination catalyzed by the ADAR family. Throughout development, A-to-I editing levels increase while ADAR expression is constant, suggesting cellular mechanisms to regulate A-to-I editing exist. Furthermore, in several disease states, ADAR expression levels are similar to the normal state, but A-to-I editing levels are altered. Therefore, understanding how these enzymes are regulated in normal tissues and misregulated in disease states is of profound importance. This chapter will both discuss how to identify A-to-I editing sites across the transcriptome and explore the mechanisms that regulate ADAR editing activity, with particular focus on the diverse types of RNA-binding proteins implicated in regulating A-to-I editing in vivo.

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Acknowledgement

This work was supported by an NIH predoctoral training grant to M.C.W. (T32 GM007757) and start-up funds from the Indiana University School of Medicine.

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  1. Department of Biology, Indiana University, Bloomington, IN, 47405, USA

    Michael C. Washburn

  2. Medical Sciences Program, Indiana University, Bloomington, IN, 47405, USA

    Heather A. Hundley

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  1. Dept of Cellular and Molecular Medicine, Institute for Genomic Medicine, La Jolla, California, USA

    Gene W. Yeo

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Washburn, M.C., Hundley, H.A. (2016). Controlling the Editor: The Many Roles of RNA-Binding Proteins in Regulating A-to-I RNA Editing. In: Yeo, G. (eds) RNA Processing. Advances in Experimental Medicine and Biology, vol 907. Springer, Cham. https://doi.org/10.1007/978-3-319-29073-7_8

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