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Abstract

In this chapter, a description of the present view of the structure of the cell membrane is presented. This includes a basic introduction to the chemistry and physics of lipids and proteins with special attention to properties that are relevant to understanding lipid-protein interactions within the membrane. It covers protein folding in membranes by describing the interaction forces involved in the process and by focusing on known cases where lipids are involved. By revisiting the fluid mosaic model for membranes the chapter finishes with a visual description of membrane structure at the nanoscale level.

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References

  • Bogdanov M, Sun J, Kaback HR, Dowhan W. A phospholipid acts as a chaperone in assembly of a membrane transport protein. Journal of Biological Chemistry. 1996;271(20):11615–8.

    Article  CAS  PubMed  Google Scholar 

  • Cymer F, von Heijne G, White SH. Mechanisms of Integral Membrane Protein Insertion and Folding. Journal of molecular biology. 2014;427(5):999–1022.

    Article  PubMed  PubMed Central  Google Scholar 

  • Danielli JF, Davson H. A contribution to the theory of permeability of thin films. Journal of cellular and comparative physiology. 1935;5(4):495–508.

    Article  CAS  Google Scholar 

  • Dowhan W, Bogdanov M. Lipid-dependent membrane protein topogenesis. Annual review of biochemistry. 2009;78:515–40.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Eisenberg D, Schwarz E, Komaromy M, Wall R. Analysis of membrane and surface protein sequences with the hydrophobic moment plot. Journal of molecular biology. 1984;179(1):125–42.

    Article  CAS  PubMed  Google Scholar 

  • Eisenberg D, Weiss RM, Terwilliger TC, Wilcox W. Hydrophobic moments and protein structure. Faraday Symposia of the Chemical Society. 1982;17:109.

    Article  Google Scholar 

  • Engelman DM, Steitz TA, Goldman A. Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins. Annual review of biophysics and biophysical chemistry. 1986;15:321–53.

    Google Scholar 

  • Escribá PV, González-Ros JM, Goñi FM, Kinnunen PKJ, Vigh L, Sánchez-Magraner L, et al. Membranes: A meeting point for lipids, proteins and therapies: Translational Medicine. Journal of cellular and molecular medicine. 2008;12(3):829–75.

    Article  PubMed  PubMed Central  Google Scholar 

  • Franks NP, Abraham MH, Lieb WR. Molecular organization of liquid n-octanol: An X-ray diffraction analysis. Journal of pharmaceutical sciences. 1993;82:466–70.

    Article  CAS  PubMed  Google Scholar 

  • Goñi FM. The basic structure and dynamics of cell membranes: an update of the Singer-Nicolson model. Biochimica et Biophysica Acta (BBA)-Biomembranes. 2014;1838(6):1467–76.

    Article  Google Scholar 

  • Gorter E, Grendel F. on Bimolecular Layers of Lipoids on the Chromocytes of the Blood. The Journal of experimental medicine. 1925;41(4):439–43.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kyte J, Doolittle RF. A simple method for displaying the hydropathic character of a protein. Journal of molecular biology. 1982;157:105–32.

    Article  CAS  PubMed  Google Scholar 

  • Martz E. Protein Explorer: Easy yet powerful macromolecular visualization. Trends in biochemical sciences. 2002;27(2):107–9.

    Article  CAS  PubMed  Google Scholar 

  • Merino-Montero S, Montero MT, Hernández-Borrell J. Effects of lactose permease of Escherichia coli on the anisotropy and electrostatic surface potential of liposomes. Biophysical chemistry. 2006;119(1):101–5.

    Article  CAS  PubMed  Google Scholar 

  • Mouritsen OG. Model answers to lipid membrane questions. Cold Spring Harbor perspectives in biology. 2011. p. 1–15.

    Google Scholar 

  • Nicolson GL. The Fluid-Mosaic Model of Membrane Structure: still relevant to understanding the structure, function and dynamics of biological membranes after more than 40 years. Biochimica et Biophysica Acta (BBA)-Biomembranes. 2014;1838(6):1451–66.

    Article  CAS  Google Scholar 

  • Nozaki Y, Tanford C. The solubility of amino acids and two glycine peptides in aqueous ethanol and dioxane solutions. Establishment of a hydrophobicity scale. Journal of Biological Chemistry. 1971;246(7):2211–7.

    CAS  PubMed  Google Scholar 

  • Picas L, Milhiet PE, Hernández-Borrell J. Atomic force microscopy: A versatile tool to probe the physical and chemical properties of supported membranes at the nanoscale. Chemistry and physics of lipids. 2012. p. 845–60.

    Google Scholar 

  • Ramachandran GN, Ramakrishnan C, Sasisekharan V. Stereochemistry of polypeptide chain configurations. J. Mol. Biol. 1963;7(1):95–9.

    Article  CAS  PubMed  Google Scholar 

  • Shimshick EJ, McConnell HM. Lateral phase separation in phospholipid membranes. Biochemistry. 1973;12(12):2351–60.

    Article  CAS  PubMed  Google Scholar 

  • Singer SJ, Nicolson GL. The fluid mosaic model of the structure of cell membranes. Science. 1972;175(23):720–31.

    Article  CAS  PubMed  Google Scholar 

  • Sun J, Frillingos S, Kaback HR. Binding of monoclonal antibody 4B1 to homologs of the lactose permease of Escherichia coli. Protein science. 1997;6(7):1503–10.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Voss J, Hubbell WL, Hernandez-Borrell J, Kaback HR. Site-directed spin-labeling of transmembrane domain VII and the 4B1 antibody epitope in the lactose permease of Escherichia coli. Biochemistry. 1997;36(49):15055–61.

    Article  CAS  PubMed  Google Scholar 

  • Wang X, Bogdanov M, Dowhan W. Topology of polytopic membrane protein subdomains is dictated by membrane phospholipid composition. The EMBO journal. 2002;21(21):5673–81.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • White SH. Membrane protein insertion: the biology-physics nexus. The Journal of general physiology. 2007;129(5):363–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • White SH, Wimley WC, Ladokhin AS, Hristova K. Protein folding in membranes: Determining energetics of peptide-bilayer interactions. Methods in enzymology. 1998;295:62–87.

    Article  CAS  PubMed  Google Scholar 

  • Wimley WC, Creamer TP, White SH. Solvation energies of amino acid side chains and backbone in a family of host-guest pentapeptides. Biochemistry. 1996;35:5109–24.

    Article  CAS  PubMed  Google Scholar 

  • Wimley WC, White SH. Experimentally determined hydrophobicity scale for proteins at membrane interfaces. Nature Structural & Molecular Biology. 1996;3(10):842–8.

    Article  CAS  Google Scholar 

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Correspondence to Jordi H. Borrell .

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Borrell, J.H., Domènech, Ò., Keough, K.M.W. (2016). Molecular Membrane Biochemistry. In: Membrane Protein – Lipid Interactions: Physics and Chemistry in the Bilayer. SpringerBriefs in Biochemistry and Molecular Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-30277-5_1

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