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Biochemistry and Physiology of Heart Mitochondrial Nitric Oxide Synthase

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Biochemistry of Oxidative Stress

Part of the book series: Advances in Biochemistry in Health and Disease ((ABHD,volume 16))

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Abstract

Heart mitochondria are the major source of reactive oxygen and nitrogen species and play a central role in cell energy provision and signaling. The NO produced by cardiac mtNOS is allowed to interact restrictedly with the co-localized effectors. NO exerts a high affinity, reversible and physiological inhibition of cytochrome c oxidase activity. A second effect of NO on the respiratory chain is accomplished through its interaction with ubiquinol-cytochrome c oxidoreductase. The ability of mtNOS to regulate mitochondrial O2 uptake and O2 − and H2O2 productions is named mtNOS functional activity. Several situations, including chronic hypoxia and ischemia-reperfusion, modify heart mtNOS activity or expression. The regulation of heart mtNOS by distinctive mitochondrial environments includes the effects of Ca2+, O2, L-arginine, NADPH, mitochondrial membrane potential (Δψ) and the metabolic states. Together, this enzyme seems to be critical during the adaptation of heart mitochondria to changes in cellular bioenergetics.

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Acknowledgments

This work was supported by research grants from the University of Buenos Aires (UBACYT 20020110100140, 20020130100731), Agencia Nacional de Promoción Científica y Tecnológica (PICT 2014-0964), and Consejo Nacional de Investigaciones Científicas y Técnicas (PIP 11220110100444).

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Authors and Affiliations

  1. Institute of Biochemistry and Molecular Medicine (IBIMOL; UBA-CONICET), Physical Chemistry Division, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina

    Tamara Zaobornyj, Darío E. Iglesias, Silvina S. Bombicino, Ivana A. Rukavina-Mikusic & Laura B. Valdez

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  1. Tamara Zaobornyj
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  2. Darío E. Iglesias
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  3. Silvina S. Bombicino
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  4. Ivana A. Rukavina-Mikusic
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  5. Laura B. Valdez
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Correspondence to Tamara Zaobornyj .

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  1. School of Medicine, University of Buenos Aires, Buenos Aires, Argentina

    Ricardo Jorge Gelpi

  2. School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina

    Alberto Boveris

  3. Instituto de Inmunología, Genética y Metabolismo (INIGEM, UBA–CONICET), Laboratorio de Metabolismo del Oxígeno, Hospital Universitario, Universidad de Buenos Aires, Buenos Aires, Argentina

    Juan José Poderoso

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Zaobornyj, T., Iglesias, D.E., Bombicino, S.S., Rukavina-Mikusic, I.A., Valdez, L.B. (2016). Biochemistry and Physiology of Heart Mitochondrial Nitric Oxide Synthase. In: Gelpi, R., Boveris, A., Poderoso, J. (eds) Biochemistry of Oxidative Stress. Advances in Biochemistry in Health and Disease, vol 16. Springer, Cham. https://doi.org/10.1007/978-3-319-45865-6_4

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