Abstract
The article demonstrates some less known principles of image build-up in diffractive microscopy and their usage in analysis unravelling the smallest localized information about the original object – an electromagnetic centroid. In fluorescence, the electromagnetic centroid is naturally at the position of the fluorophore. The usage of an information-entropic variable – a point divergence gain – is demonstrated for finding the most localized position of the object’s representation, generally of the size of a voxel (3D pixel). These spatial pixels can be qualitatively classified and used for reconstruction of the 3D structures with precision comparable with electron microscopy.
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Acknowledgments
This work was supported by the Ministry of Education, Youth and Sports of the Czech Republic – projects CENAKVA (No. CZ.1.05/2.1.00/01.0024), CENAKVA II (No. LO1205 under the NPU I program), The CENAKVA Centre Development (No. CZ.1.05/2.1.00/19.0380) – and by the CZ-A AKTION programme.
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Rychtáriková, R., Steiner, G., Fischer, M.B., Štys, D. (2017). Information Limits of Optical Microscopy: Application to Fluorescent Labelled Tissue Section. In: Rojas, I., Ortuño, F. (eds) Bioinformatics and Biomedical Engineering. IWBBIO 2017. Lecture Notes in Computer Science(), vol 10208. Springer, Cham. https://doi.org/10.1007/978-3-319-56148-6_43
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DOI: https://doi.org/10.1007/978-3-319-56148-6_43
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