Abstract
Müller cells provide support to photoreceptors under many conditions of stress and degeneration. Leukemia inhibitory factor is known to be expressed in Müller cells, which is necessary to promote photoreceptor survival in stress. We hypothesize that Müller cells that express LIF are undergoing other biological processes or functions which may benefit photoreceptors in disease. In this study, we analyze an existing single Müller cell microarray dataset to determine which processes are upregulated in Müller cells that express LIF, by correlating LIF expression to the expression of other genes using a robust correlation method. Some enriched processes include divalent inorganic cation homeostasis, negative regulation of stem cell proliferation, and gamma-glutamyl transferase activity.
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Acknowledgments
We thank K. Roesch and the Cepko group for developing the dataset that made this study possible. This study was supported by NIH grant R01 EY016459-11, training grant EY007132, Foundation Fighting Blindness, and an unrestricted departmental grant from Research to Prevent Blindness.
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Supplemental Fig. 59.1
Hooper cell differentiation LIF protein coding region (TIFF 29160 kb)
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Hooper, M.J., Ash, J.D. (2018). Müller Cell Biological Processes Associated with Leukemia Inhibitory Factor Expression. In: Ash, J., Anderson, R., LaVail, M., Bowes Rickman, C., Hollyfield, J., Grimm, C. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 1074. Springer, Cham. https://doi.org/10.1007/978-3-319-75402-4_59
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DOI: https://doi.org/10.1007/978-3-319-75402-4_59
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