Abstract
Copper is one of the essential micronutrients for growth of paddy plant. High concentration of copper in the soils causes oxidative stress to paddy plant. In order to overcome from this oxidative stress, plants have several anti-oxidative responses. This research aimed to study the effects of Cu treated water on plant growth, Cu accumulation, superoxide dismutase (SOD) activity and total phenolic compounds concentration in paddy (Oryza sativa L. ‘Cempo Merah’). Copper at concentrations of 0mg/L, 100mg/L, and 300mg/L was added to the water irrigation after 28 days cultivated. Then, the samples of soils and leaves were collected four times, i.e. (0, 4, 72, and 168) h post-Cu treated. The accumulation of Cu in the soils and paddy leaves was analyzed by using flame-AAS. The superoxide dismutase (SOD) activity was analyzed by using principal of NBT’s photo-inhibition method and total phenolic compounds were analyzed by using Folin-Ciocalteau method. The variables observed were the plants’ growth parameters, the number of copper concentration in the soil and the paddy leaves post-treated, the SOD activity, and the total phenolic compounds concentration in paddy. The result showed that the addition of Cu2+ at various concentrations in the water irrigation inhibited the plant growth, increase the concentration of Cu in the soil and the paddy leaves. The increase in the concentration of Cu2+ also increased the SOD activity and the total phenolic compounds concentration in ‘Cempo Merah’ leaves linearly.
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Abbreviations
- BSA:
-
Bovine Serum Albumin
- LPDP:
-
Lembaga Pengelola Dana Pendidikan
- LPPT:
-
Laboratorium Penelitian dan Pengujian Terpadu
- ROS:
-
Reactive Oxygen Species
- SOD:
-
Superoxide Dismutase
- TPC:
-
Total Phenolic Compounds
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Acknowledgments
The author acknowledges financial support of the Indonesian Government through the funding from Indonesia Endowment Fund for Education (LPDP) Ministry of Finance of Republic of Indonesia.
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Aryani, R.D., Nugroho, A.P., Purwestri, Y.A. (2018). Effects of Copper on Accumulation, Plant Growth, Superoxide Dismutase Activity and Total Phenolic Compounds in Paddy (Oryza sativa L. ‘Cempo Merah’) Leaves. In: Sukartiko, A., Nuringtyas, T., Marliana, S., Isnansetyo, A. (eds) Proceeding of the 2nd International Conference on Tropical Agriculture. Springer, Cham. https://doi.org/10.1007/978-3-319-97553-5_20
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DOI: https://doi.org/10.1007/978-3-319-97553-5_20
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