Potentials of a Ribozyme-Based Gene Discovery System

Abstract

The recent advent of genomic tools has provided us with very powerful ways for the identification of functional genes, thus enhancing our understanding of the molecular basis of both normal and disease phenotypes. Now that sequence information is available for many genomes, a simpler and more definitive technology for the rapid identification of functional genes is a current focus of interest. A simple screening system based on the catalytic activity of ribozymes, whose target specificities are coupled with loss-of-function mutants, has been developed to isolate key genes involved in a defined phenotype. The system was validated for functional gene screens including apoptosis, transformation, metastasis, and muscle and normal differentiation. This system should be applicable to the identification of functional genes involved in many cellular processes and diseases.