Abstract
The dynamic behaviour of cells is a consequence of the coordinated and elaborate interactions between complexes of macromolecules that constitute their formed structures or organelles. Our understanding of basic cell structure and function has been greatly aided by the identification of proteins at the ultrastructural level. Advances in molecular biology, organic chemistry, and materials science have recently led to the creation of several new classes of fluorescent probes for live cell imaging and correlated light and electron microscopy (EM; Fig. 1; [1,2]). Here we show the application of (i) small organic fluorescent dyes, (ii) nanocrystals (“quantum dots”), (iii) fluorescent proteins, including the optimized mCherry, and (iv) genetic encoded tetracysteine tags complexed with biarsenical dyes (FlAsH and ReAsH), to determine protein dynamics and ultrastructural localization of proteins and organelle structures.
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References
Giepmans BNG, Adams SR, Ellisman MH & Tsien RY (2006). The fluorescent toolbox for assessing protein location and function. Science 312, 217–224.
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Giepmans, B.N.G., Gaietta, G.M., Deerinck, T.J., Adams, S.R., Tsien, R.Y., Ellisman, M.H. (2008). Golgi twins in mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy. In: Aretz, A., Hermanns-Sachweh, B., Mayer, J. (eds) EMC 2008 14th European Microscopy Congress 1–5 September 2008, Aachen, Germany. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-85228-5_169
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DOI: https://doi.org/10.1007/978-3-540-85228-5_169
Publisher Name: Springer, Berlin, Heidelberg
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