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Raman Microscopy and Imaging: Applications to Skin Pharmacology and Wound Healing

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Emerging Raman Applications and Techniques in Biomedical and Pharmaceutical Fields

Part of the book series: Biological and Medical Physics, Biomedical Engineering ((BIOMEDICAL))

Abstract

The utility of confocal Raman microscopy to study biological events in skin is demonstrated with three examples. (i) monitoring the spatial and structural differences between native and cultured skin, (ii) tracking the permeation and biochemical transformation in skin of a Vitamin E derivative and (iii) tracking the spatial distribution of three major skin proteins (keratin, collagen, and elastin) during wound healing in an explant skin model.

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References

  1. C. Xiao, D.J. Moore, M.E. Rerek, C.R. Flach, R. Mendelsohn, J. Invest. Dermatol. 124, 622 (2005)

    Google Scholar 

  2. G. Zhang, D.J. Moore, R. Mendelsohn, C.R. Flach, J. Invest. Dermatol. 126, 1088 (2006)

    Google Scholar 

  3. G. Zhang, D.J. Moore, K.B. Sloan, C.R. Flach, R. Mendelsohn, J. Invest. Dermatol. 127, 1205 (2007)

    Google Scholar 

  4. G. Zhang, D.J. Moore, C.R. Flach, R. Mendelsohn, Anal. Bioanal. Chem. 387, 1591 (2007)

    Article  Google Scholar 

  5. G. Zhang, C.R. Flach, R. Mendelsohn, J. Control. Release 123, 141 (2007)

    Article  Google Scholar 

  6. K.L.A. Chan, G. Zhang, M. Tomic-Canic, O. Stojadinovic, B. Lee, C.R. Flach, R. Mendelsohn, J. Cell. Mol. Med. 12, 2145 (2008)

    Google Scholar 

  7. P.M. Elias, J. Control Release 15, 199 (1991)

    Article  Google Scholar 

  8. B. Forslind, Acta Derm. Venerol. 74, 1 (1994)

    Google Scholar 

  9. P.J. Caspers, G.W. Lucassen, R. Wolthuis, H.A. Bruining, G.J. Puppels, Biospectroscopy 4, S31 (1998)

    Article  Google Scholar 

  10. P.J. Caspers, A.C. Williams, E.A. Carter, H.G. Edwards, B.W. Barry, H.A. Bruining, G.J. Puppels, Pharm. Res. 19, 1577 (2002)

    Article  Google Scholar 

  11. P.J. Caspers, G.W. Lucassen, H.A. Bruining, G.J. Puppels, J. Raman Spectrosc. 31, 813 (2000)

    Article  ADS  Google Scholar 

  12. P.J. Caspers, G.W. Lucassen, E.A. Carter, H.A. Bruining, G.J. Puppels, J. Invest. Dermatol. 116, 434 (2001)

    Google Scholar 

  13. P.D.A. Pudney, M. Mélot, P.J. Caspers, A. Van Der Pol, G.J. Puppels, Appl. Spectrosc. 61, 804 (2007)

    Article  ADS  Google Scholar 

  14. A. Nijssen, T.C. Bakker Schut, F. Heule, P.J. Caspers, D.P. Hayes, M. Neumann, G.J. Puppels, J. Invest. Dermatol. 119, 64 (2002)

    Google Scholar 

  15. J. Wohlrab, A. Vollmann, S. Wartewig, W.C. Marsch, R. Neubert, Biopolymers 62, 141 (2001)

    Article  Google Scholar 

  16. J. Choi, J. Choo, H. Chung, D.-G. Gweon, J. Park, H.J. Kim, S. Park, C.-H. Oh, Biopolymers 77, 264 (2005)

    Article  Google Scholar 

  17. M. Gniadecka, P.A. Philipsen, S. Sigurdsson, S. Wessel, O.F. Nielsen, D.H. Christensen, J. Hercogova, K. Rossen, H.K. Thomsen, R. Gniadecki, L.K. Hansen, H.C. Wulf, J. Invest. Dermatol. 122, 443 (2004)

    Google Scholar 

  18. A. Nijssen, K. Maquelin, L.F. Santos, P.J. Caspers, T.C. Bakker Schut, J.C. den Hollander, M.H.A. Neumann, G.J. Puppels, J. Biomed. Opt. 12, 034004 (2007)

    Article  ADS  Google Scholar 

  19. E. Ly, O. Piot, A. Durlach, P. Bernard, M. Manfait, Appl. Spectrosc. 62, 1088 (2008)

    Article  ADS  Google Scholar 

  20. N.S. Eikje, K. Aizawa, Y. Ozaki, in Biotechnology Annual Review, vol. 11, ed. by M.R. El-Gewely (Elsevier, Amsterdam, 2005), p. 191

    Google Scholar 

  21. N.J. Everall, Appl. Spectrosc. 54, 1515 (2000)

    Article  ADS  Google Scholar 

  22. N.J. Everall, Appl. Spectrosc. 54, 773 (2000)

    Article  ADS  Google Scholar 

  23. N. Everall, Appl. Spectrosc. 62, 591 (2008)

    Article  ADS  Google Scholar 

  24. T.E. Bridges, M.P. Houlne, J.M. Harris, Anal. Chem. 76, 576 (2004)

    Article  Google Scholar 

  25. A.M. Macdonald, A.S. Vaughan, P.J. Wyeth, J. Raman Spectrosc. 36, 185 (2005)

    Article  ADS  Google Scholar 

  26. B.W. Barry, H.G.M. Edwards, A.C. Williams, J. Raman Spectrosc. 23, 641 (1992)

    Article  ADS  Google Scholar 

  27. J.L. Lippert, W.L. Peticolas, Proc. Natl. Acad. Sci. U S A 68, 1572 (1971)

    Article  ADS  Google Scholar 

  28. J.J. Baraga, M.S. Feld, R.P. Rava, Proc. Natl. Acad. Sci. U S A 89, 3473 (1992)

    Article  ADS  Google Scholar 

  29. R.D. Pensack, B.B. Michniak, D.J. Moore, R. Mendelsohn, Appl. Spectrosc. 60, 1399 (2006)

    Article  ADS  Google Scholar 

  30. H. Deng, V.A. Bloomfield, J.M. Benevides, G.J. Thomas, Biopolymers 50, 656 (1999)

    Article  Google Scholar 

  31. W.L. Peticolas, W.L. Kubasek, G.A. Thomas, M. Tsuboi, in Biological Applications of Raman Spectroscopy, vol. 1, ed. by T.G. Spiro (Wiley, New York, 1987), p. 81

    Google Scholar 

  32. R.C. Lord, G.J. Thomas, Spectrochim. Acta A 23, 2551 (1967)

    Article  ADS  Google Scholar 

  33. A. Tfayli, O. Piot, F. Draux, F. Pitre, M. Manfait, Biopolymers 87, 261 (2007)

    Article  Google Scholar 

  34. M.N. Siamwiza, R.C. Lord, M.C. Chen, T. Takamatsu, I. Harada, H. Matsuura, T. Shimanouchi, Biochemistry 14, 4870 (1975)

    Article  Google Scholar 

  35. M. Rangarajan, J.L. Zatz, J. Cosmet. Sci. 52, 225 (2001)

    Google Scholar 

  36. W. Baschong, C. Artmann, D. Hueglin, J. Roeding, J. Cosmet. Sci. 52, 155 (2001)

    Google Scholar 

  37. A. Mavon, V. Raufast, D. Redoulés, J. Control. Release 100, 221 (2004)

    Article  Google Scholar 

  38. G.C. Gurtner, S. Werner, Y. Barrandon, M.T. Longaker, Nature 453, 314 (2008)

    Article  ADS  Google Scholar 

  39. P.A. Coulombe, J. Invest. Dermatol. 121, 219 (2003)

    Article  Google Scholar 

  40. I.M. Freedberg, M. Tomic-Canic, M. Komine, M. Blumenberg, J. Invest. Dermatol. 116, 633 (2001)

    Article  Google Scholar 

  41. A.J. Singer, R.A.F. Clark, N. Engl. J. Med. 341, 738 (1999)

    Article  Google Scholar 

  42. M. Tomic-Canic, S.W. Mamber, O. Stojadinovic, B. Lee, N. Radoja, J. McMichael, Wound Repair Regen. 15, 71 (2007)

    Article  Google Scholar 

  43. H. Brem, O. Stojadinovic, R.F. Diegelmann, H. Entero, B. Lee, I. Pastar, M. Golinko, H. Rosenberg, M. Tomic-Canic, Mol. Med. 13, 30 (2007)

    Article  Google Scholar 

  44. B.G. Frushour, J.L. Koenig, Biopolymers 14, 379 (1975)

    Article  Google Scholar 

  45. L. Debelle, A.J.P. Alix, M.-P. Jacob, J.-P. Huvenne, M. Berjot, B. Sombret, P. Legrand, J. Biol. Chem. 270, 26099 (1995)

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Newark College, Rutgers University, 73 Warren Street, Newark, NJ, 17102, USA

    Carol R. Flach, Guojin Zhang & Richard Mendelsohn

Authors
  1. Carol R. Flach
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  2. Guojin Zhang
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  3. Richard Mendelsohn
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Corresponding author

Correspondence to Carol R. Flach .

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Editors and Affiliations

  1. Science & Technology Facilities Council, Central Laser Facility, Didcot, Oxon., OX11 0QX, United Kingdom

    Pavel Matousek

  2. Dept. Chemistry, University of Michigan, Ann Arbor, 48109-1055, U.S.A.

    Michael D. Morris

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Flach, C.R., Zhang, G., Mendelsohn, R. (2010). Raman Microscopy and Imaging: Applications to Skin Pharmacology and Wound Healing. In: Matousek, P., Morris, M. (eds) Emerging Raman Applications and Techniques in Biomedical and Pharmaceutical Fields. Biological and Medical Physics, Biomedical Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02649-2_15

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  • DOI: https://doi.org/10.1007/978-3-642-02649-2_15

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  • Print ISBN: 978-3-642-02648-5

  • Online ISBN: 978-3-642-02649-2

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