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Specific Staining Reactions

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Quantitative Thin-Layer Chromatography

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Abstract

An undoubted advantage of TLC is the simplicity of qualifying a separation. Coloured compounds can be seen by eye. Compounds absorbing only below 400 nm can be visualized by incorporating a fluorescent dye in the layer. Illuminating the plate with light of 256 nm will induce fluorescence. A sample zone covers the dye and inhibits fluorescence, so the zone is visible as a dark band. Illuminating the plate with light of 366 nm can identify substances with native fluorescence. Under these conditions you can observe most of the fluorescent substances by eye.

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Authors and Affiliations

  1. FB Maschinenbau und Verfahrenstechnik, Hochschule Offenburg, Badstr. 24, 77652, Offenburg, Germany

    Prof. Dr. Bernd Spangenberg

  2. Dept. Chemistry, Wayne State University, Cass Avenue 5101, 48202, Detroit, Michigan, USA

    Colin F. Poole

  3. Hasenfeld 7, 66132, Bischmisheim, Saarland, Germany

    Christel Weins

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  1. Prof. Dr. Bernd Spangenberg
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  2. Colin F. Poole
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  3. Christel Weins
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Correspondence to Bernd Spangenberg .

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Spangenberg, B., Poole, C.F., Weins, C. (2010). Specific Staining Reactions. In: Quantitative Thin-Layer Chromatography. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-10729-0_7

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