Abstract
Poly (N-isopropylacrylamide) (PNIPAAm) is one of the most popular thermoresponsive polymers, which shows dramatic and reversible phase transition behavior in water. Therefore two strategies for the design of temperature-sensitive enzyme model based on PNIPAAm derivatives are presented: 1) A temperature-sensitive block copolymer (PAAm-b-PNIPAAm-Te) with a glutathione peroxidase-like active site was synthesized via ATRP. 2) An artificial bifunctional enzyme with both superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities was constructed by the self-assembly of a porphyrin core with four suspensory adamantyl moieties and b-cyclodextrin-terminated temperature-sensitive copolymer (β-CD-PEG-b-PNIPAAm-Te) through host–guest interaction in aqueous solution. With increasing temperature, the PNIPAAm chain becoming hydrophobic leads to a change in the self-assembly structure of the polymer, which plays a key role in modulating the catalytic activity. And two new artificial enzymes were found to exhibit the highest enzymatic catalytic efficiency in close to physical temperature.
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Lin, T., Lin, J., Huang, X., Liu, J. (2011). Artificial Enzyme Construction with Temperature Sensitivity. In: Lin, S., Huang, X. (eds) Advances in Computer Science, Environment, Ecoinformatics, and Education. CSEE 2011. Communications in Computer and Information Science, vol 214. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-23321-0_40
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DOI: https://doi.org/10.1007/978-3-642-23321-0_40
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