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Combined Model-Based and Region-Adaptive 3D Segmentation and 3D Co-Localization Analysis of Heterochromatin Foci

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Bildverarbeitung für die Medizin 2012

Part of the book series: Informatik aktuell ((INFORMAT))

Abstract

The nuclear organization of euchromatin and heterochromatin is important for genome regulation and cell function. Therefore, the analysis of heterochromatin formation and maintenance is an important topic in biological research. We introduce an automatic approach or analyzing heterochromatin foci in 3D multi-channel fluorescence microscopy images. The approach combines model-based segmentation with region-adaptive segmentation and performs a 3D co-localization analysis in different nuclear regions. Our approach has been successfully applied to 275 3D two-channel fluorescence microscopy images of mouse fibroblast cells.

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Author information

Authors and Affiliations

  1. Biomedical Computer Vision Group, Univ., BioQuant, IPMB, and DKFZ Heidelberg, Heidelberg, Deutschland

    Simon Eck, Karl Rohr & Stefan Wörz

  2. Research Group Genome Organization & Function, DKFZ and BioQuant, Heidelberg, Heidelberg, Deutschland

    Katharina Müller-Ott & Karsten Rippe

Authors
  1. Simon Eck
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  2. Karl Rohr
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  3. Katharina Müller-Ott
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  4. Karsten Rippe
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  5. Stefan Wörz
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Corresponding author

Correspondence to Simon Eck .

Editor information

Editors and Affiliations

  1. Charité Campus Benjamin Franklin, Inst. Medizinische Informatik,, Universitätsklinikum Berlin, Hindenburgdamm 30, Berlin, 12200, Berlin, Germany

    Thomas Tolxdorff

  2. Inst. Medizinische Informatik, RWTH Aachen, Pauwelsstr. 30, Aachen, 52074, Germany

    Thomas Martin Deserno

  3. , Institut für Medizinische Informatik, Universität zu Lübeck, Ratzeburger Allee 160, Lübeck, 23562, Germany

    Heinz Handels

  4. Abt. Med./Biol.Inform./H0100, Deutsches Krebsforschungzentrum (DKFZ), Im Neuenheimer Feld 280, Heidelberg, 69120, Germany

    Hans-Peter Meinzer

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© 2012 Springer-Verlag Berlin Heidelberg

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Eck, S., Rohr, K., Müller-Ott, K., Rippe, K., Wörz, S. (2012). Combined Model-Based and Region-Adaptive 3D Segmentation and 3D Co-Localization Analysis of Heterochromatin Foci. In: Tolxdorff, T., Deserno, T., Handels, H., Meinzer, HP. (eds) Bildverarbeitung für die Medizin 2012. Informatik aktuell. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-28502-8_4

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