Abstract
The objective of this exercise is to sequence the pUC18c cloning vector. It is divided into the following major parts: (a) downloading the cloning vector, (b) run a virtual sequencing program, and (c) assembling the virtual sequences obtained from sequencing with the TIGR Assembler. The quality of a particular assembly is visualized with a dot plot. The ultimate goal is to understand the effect of sequence coverage and sequence fragment length on the assembly process. This exercise uses a virtual sequencer, the TIGR Assembler, Dotter, R, and shell programming.
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© 2013 Springer-Verlag Berlin Heidelberg
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Wünschiers, R. (2013). Virtual Sequencing of pUC18c. In: Computational Biology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-34749-8_20
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DOI: https://doi.org/10.1007/978-3-642-34749-8_20
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Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-34748-1
Online ISBN: 978-3-642-34749-8
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