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Optimization of Diosgenin Production by Mixed Culture Using Response Surface Methodology

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 249))

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Abstract

Optimization of four process parameters was attempted using Box-Behnken design for production of diosgenin by a mixed culture with Aspergillus oryzae, Phanerochaete chrysosporium, and Aspergillus niger. Maximum diosgenin yield of 42.89 ± 0.53 mg/g was obtained after optimization of culture conditions such as Dioscorea zingiberensis C.H.Wright (DZW) concentration 42.98 g/l, inoculum size 1.73 ml of spore suspension (1: 2: 3 ratio of A. oryzae, P. chrysosporium, and A. niger), initial pH 5.0, and cultivation time 5.27 days (127 h) and incubated at 30 °C on a rotary shaker set at 180 r/min as mixed culture.

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Acknowledgments

This work was financially supported by the Tianjin Science and Technology Support Plan Project (10ZCKFSH0060) and the Natural Science Foundation of China (No. 20906071).

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Authors and Affiliations

  1. Key Laboratory of Industrial Microbiology, Ministry of Education, National Engineering Laboratory for Industrial Enzymes, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Jinxia Xie, Xing Xu & Songtao Bie

Authors
  1. Jinxia Xie
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  2. Xing Xu
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  3. Songtao Bie
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Corresponding author

Correspondence to Jinxia Xie .

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Editors and Affiliations

  1. College of Bioengineering, Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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Xie, J., Xu, X., Bie, S. (2014). Optimization of Diosgenin Production by Mixed Culture Using Response Surface Methodology. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_12

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  • DOI: https://doi.org/10.1007/978-3-642-37916-1_12

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37915-4

  • Online ISBN: 978-3-642-37916-1

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