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Production of Artemisinin In Planta and in Microbial Systems Need Not Be Mutually Exclusive

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Artemisia annua - Pharmacology and Biotechnology

Abstract

Artemisia annua L. is an innocuous medicinal plant that is suddenly found at the forefront of global efforts aimed at the eradication of malaria. The plant is also seen as an effective treatment against several other infectious diseases and human cancer cell lines, and this has been correlated with its richness in several bioactive compounds including artemisinin, other sesquiterpenes, and antioxidants. Undoubtedly, this is a development that has drastically increased artemisinin demand worldwide. Up until now, A. annua L. remains the only commercial source for the supply of this vital antimalarial drug to the international market. Recent advances in biotechnology, however, such as have been demonstrated in the production of isoprenoid precursors of artemisinin in yeast, and bacteria are feasible complementary strategies that would help reduce artemisinin cost in the future. The key genes encoding for enzymes regulating the biosynthesis of artemisinin in planta are fully understood to enable metabolic engineering of the pathway, and results from pilot genetic engineering studies in microbial strains thus far are very inspiring. This current treatise, therefore, explores the status of artemisinin and other plant metabolites for use in both human and animal healthcare and highlights the implications of in planta production of artemisinin in comparison with that from synthetic biology. Overall, these two methods need not be mutually exclusive and can be made complementary to each other depending on the location of production. Aside from artemisinin, required for saving the lives of countless patients in malaria-stricken societies, the plant also contains several other secondary metabolites with enormous benefits in the promotion of human and animal health. Consequently, keeping in planta production of artemisinin would play a pivotal role in providing artemisinin for ACTs as well as maintaining profit margins to local and regional economies in countries where malaria is endemic, especially in Africa where A. annua has been cultivated for the past 10 years.

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References

  • Abate S, Damtew Z, Mengesha B (2011) Artemisia annua as an alternative potential weed control option. Afr J Food Agri Nutrition Develop 11:1–6

    Google Scholar 

  • Adaniya S, Shira D (2001) In vitro induction of tetraploid ginger (Zingiber officinalis Roscoe) and its pollen fertility and germinability. Sci Hort 88:277–287

    Article  Google Scholar 

  • Alin MH, Bjorkman A (1994) Concentration and time dependency of artemisinin efficacy against Plasmodium falciparum in vitro. Am J Trop Med Hyg 50:771–776

    PubMed  CAS  Google Scholar 

  • Almeida GF, Horsted K, Thamsborg SM, Kyvsgaard NC, Ferreira JFS, Hermansen JE (2012) Use of Artemisia annua as a natural coccidiostat in free-range broilers and its effects on infection dynamics and performance. Vet Parasitol 186:178–187. doi http://dx.doi.org/10.1016/j.vetpar.2011.11.058

  • Arsenault PR, Wobbe KK, Weathers PJ (2008) Recent advances in artemisinin production through heterologous expression. Curr Med Chem 15:2886–2896

    Article  PubMed  CAS  Google Scholar 

  • Assured Artemisinin Supply System (A2S2) (2012) Production cycle: from artemisia to ACT, 26 Jan 2012: http://www.a2s2.org/index.php?id=50

  • Bhakuni RS, Jain DC, Sharma RP, Kumar S (2001) Secondary metabolites of Artemisia annua and their biological activity. Curr Sci 80:35–48

    CAS  Google Scholar 

  • Bowles D, Smallwood M, Graham I (2008) Fast track breeding of A. annua. Ph.D symposium of the Zurich-Basel Plant Science Center, 6 June, Switzerland

    Google Scholar 

  • Brisibe EA, Daniel JU (2013) Efficacy of fresh whole leaf preparations of Artemisia annua against uncomplicated falciparum malaria based on polymerase chain reaction fingerprinting. Afr J Biotechnol (under review)

    Google Scholar 

  • Brisibe EA, Udensi O, Chukwurah PN, de Magalhäes PM, Figueira GM, Ferreira JFS (2012) Adaptation and agronomic performance of Artemisia annua L. under lowland humid tropical conditions. Ind Crops Prod 39:190–197. doi:http://dx.doi.org/10.1016/j.indcrop.2012.02.018

  • Brisibe EA, Brisibe F, Agba D, Abang AE (2011a) Antihyperglycaemic activity and haematological efficacy of Artemisia annua leaves as dietary inclusions in albino rats. Molecules 16: 1-x manuscripts; doi:10.3390/molecules160x000x (Accepted for publication)

  • Brisibe EA, Adugbo SE, Ekanem U, Brisibe F, Figueira GM (2011b) Controlling bruchid pests of stored cowpea seeds with dried leaves of Artemisia annua and two other common botanicals. Afr J Biotechnol 10:9586–9592

    Google Scholar 

  • Brisibe EA, Umoren UE, Brisibe F, Magalhaes PM, Ferreira JFS, Luthria D, Wu X, Prior P (2009) Nutritional characterization and antioxidant capacity of different tissues of Artemisia annua L. Food Chem 115:1240–1426

    Article  CAS  Google Scholar 

  • Brisibe EA, Umoren UE, Owai PU, Brisibe F (2008a) Dietary inclusion of dried Artemisia annua leaves for management of coccidiosis and growth enhancement in chicken. Afr J Biotechnol 7:4083–4092

    CAS  Google Scholar 

  • Brisibe EA, Uyoh EA, Brisibe F, Magalhäes PM, Ferreira JFS (2008b) Building a golden triangle for the production and use of artemisinin derivatives against falciparum malaria in Africa. Afr J Biotechnol 7:4884–4896

    CAS  Google Scholar 

  • Brisibe EA (2006) Challenges and opportunities in the local production of artemisinin-based combination therapies against malaria in Nigeria. J Pharm Sci Pharm Pract 8:49–59

    Google Scholar 

  • Cheeseman IH, Miller BA, Nair S, Nkhoma S, Tan A, Tan JC, Saai SA, Phyo AP, Moo CL, Lwin KM, McGready R, Ashley E, Imwong M, Stepniewska K, Yi P, Dondorp AM, Mayxay M, Newton PN, White NJ, Nosten F, Ferdig MT, Anderson TJC (2012) A major genome region underlying artemisinin resistance in malaria. Science 336:79–82

    Google Scholar 

  • Chen DH, Ye HC, Li GF (2000) Expression of a chimeric farnesyl diphosphate synthase gene in A. annua L. transgenic plants via Agrobacterium tumefaciens-mediated transformation. Plant Sci 155:179–185

    Article  PubMed  CAS  Google Scholar 

  • Cherian G, Orr IA, Burke IC, Pan W (2013) Feeding Artemisia annua alters digesta pH and muscle lipid oxidation products in broiler chickens. Poult Sci 92:1085–1090

    Article  PubMed  CAS  Google Scholar 

  • Debrunner N, Dvorak V, Magalhaes P, Delabays N (1996) Selection of genotypes of A. annua L. for the agricultural production of artemisinin. In: Pank F (ed) international symposium on breeding research on medicinal plants, Quedlinburg, pp 222–225

    Google Scholar 

  • Delabays N, Darbellay C, Galland N (2002) Variation and heritability of artemisinin content of A. annua L. In: Wright CW (ed) Artemisia. Taylor and Francis, London, pp 197–209

    Google Scholar 

  • Delabays N, Simonnet X, Gaudin M (2001) The genetics of artemisinin content in A. annua L. and the breeding of high yielding cultivars. Curr Med Chem 8:1795–1801

    Article  PubMed  CAS  Google Scholar 

  • Delabays N (1994) La Domestication et L’Amelioration Genetique De L’Artemisia annua Dans Le Cadre Du Developpement D’un Nouveau Medicament Contre La Malaria. In: International conference on aromatic and medicinal plants, pp 1–11

    Google Scholar 

  • Drăgan L, Titilincu A, Dan I, Dunca I, Drăgan M, Mircean V (2010) Effects of A. annua and Pimpinella anisum on Eimeria tenella (Phylum Apicomplexa) low infection in chickens. Sci Parasitol 11:77–82

    Google Scholar 

  • De Ridder S, van der Kooy F, Verpoorte R (2008) Artemisia annua as a self-reliant treatment for malaria in developing countries. J Ethnopharmacol 120:302–314. doi:10.1016/j.jep.2008.09.017

    Article  PubMed  Google Scholar 

  • Efferth T, Herrmann F, Tahrani A, Wink M (2011) Cytotoxic activity of secondary metabolites derived from Artemisia annua L. towards cancer cells in comparison to its designated active constituent artemisinin. Phytomedicine 18:959–969

    Article  PubMed  CAS  Google Scholar 

  • Efferth T, Romero MR, Wolf DG, Stamminger T, Marin JJG, Marschall M (2008) The antiviral activities of artemisinin and artesunate. Clin Infect Dis 47:804–811

    Article  PubMed  CAS  Google Scholar 

  • Efferth T, Dunstan H, Sauerberry A, Miyachi H, Chitambar CR (2001) Antimalarial artesunate is also active against cancer. Int J Oncol 18:767–773

    PubMed  CAS  Google Scholar 

  • Ekanem A, Brisibe EA (2010) Effects of ethanol extract of Artemisia annua L. against monogenean parasites of Heterobranchus longifilis. Parasitol Res 106:1135–1139. doi:10.1007/s00436-010-1787-0

    Article  PubMed  Google Scholar 

  • Elfawal MA, Towler MJ, Reich NG, Golenbock D, Weathers PJ, Rich SM (2012) Dried whole plant Artemisia annua as an antimalarial therapy. PLoS ONE 7:e52746. doi:10.1371/journal.pone.0052746

    Article  PubMed  CAS  Google Scholar 

  • Elford BC, Roberts MF, Phillipson JD, Wilson RJ (1987) Potentiation of the antimalarial activity of qinghaosu by methoxylated flavones. Trans R Soc Trop Med Hyg 81:434–436. doi:10.1016/0035-9203(87)90161-1

    Article  PubMed  CAS  Google Scholar 

  • Ellman A (2010) Cultivation of Artemisia annua in Africa and Asia. Outlooks Pest Manage 21:84–88

    Article  Google Scholar 

  • Ferreira JFS, Luthria DL, Sasaki T, Heyerick A (2010) Flavonoids from A. annua L. as antioxidants and their potential synergism with artemisinin against malaria and cancer. Molecules 15:3135–3170

    Article  PubMed  CAS  Google Scholar 

  • Ferreira JFS, Janick J (1995) Floral morphology of A. annua with special reference to trichomes. Int J Plant Sci 156:807–815

    Article  Google Scholar 

  • Ferreira JFS, Laughlin JC, Delabays N, Magalhães PM (2005) Cultivation and genetics of A. annua L. for increased production of the antimalarial artemisinin. Plant Genet Res 3:206–229

    Article  CAS  Google Scholar 

  • Ferreira JFS (2009) Artemisia species in small ruminant production: their potential antioxidant and anthelmintic effects. In: Morales M (ed) Appalachian workshop and research update: improving small ruminant grazing practices. Mountain State University/USDA, Beaver, WV, pp 53–70

    Google Scholar 

  • Ferreira JFS (2008) Seasonal and post-harvest accumulation of artemisinin, artemisinic acid, and dihydroartemisinic acid in three accessions of A. annua cultivated in West Virginia, USA. Planta Medica 74:310–311(Abstract only)

    Google Scholar 

  • Ferreira JFS (2007) Nutrient deficiency in the production of artemisinin, dihydroartemisinic acid, and artemisinic acid in A. annua L. J Agric Food Chem 55:1686–1694

    Article  PubMed  CAS  Google Scholar 

  • Ferreira JFS, Gonzalez JM (2009) Analysis of underivatized artemisinin and related sesquiterpene lactones by high-performance liquid chromatography with ultraviolet detection. Phytochem Anal 20:91–97

    Article  PubMed  CAS  Google Scholar 

  • Gilbert B, Alves LF (2003) Synergy in plant medicines. Curr Medicin Chem 10:13–20

    Article  CAS  Google Scholar 

  • Gordi T, Huong DX, Hai TN, Nieu NT, Ashton M (2002) Artemisinin pharmacokinetics and efficacy in uncomplicated-malaria patients treated with two different dosage regimens. Antimicrob Agents Chemother 46:1026–1031

    Article  PubMed  CAS  Google Scholar 

  • Graham IA, Besser K, Blumer S, Branigan CA, Czechowski T, Elias L, Guterman I, Harvey D, Isaac PG, Khan AM, Larson TR, Li Y, Pawson T, Penfield T, Rae AM, Rathbone DA, Reid S, Ross J, Smallwood MF, Segura V, Townsend T, Vyas D, Winzer T, Bowles D (2010) The genetic map of A. annua L. identifies loci affecting yield of the antimalarial drug artemisinin. Science 327:328–331. doi:10.1126/science.1182612

    Article  PubMed  CAS  Google Scholar 

  • Griesbach RJ, Kamo KK (1996) The effect of induced polyploidy on the flavonoids of Petunia ‘Mitchell’. Phytochemistry 42:361–363

    Article  CAS  Google Scholar 

  • Hale H, Keasling JD, Renninger N, Diagana TT (2007) Microbially derived artemisinin: A biotechnology solution to the global problem of access to affordable antimalarial drugs. Am J Trop Med Hyg 77:198–202

    PubMed  Google Scholar 

  • Hart SP, Ferreira JFS, Wang Z (2007) Efficacy of wormwoods (Artemisia spp.) as an anthelmintic in goats. J An Sci 86:92

    Google Scholar 

  • Heemskerk W, Schallig H, Piters BD (2006) The world of Artemisia in 44 questions, Royal Tropical Institute (commissioned by the Netherlands Directorate-General for International Cooperation, Ministry of Foreign Affairs), Netherlands, March, p 85 (www.kit.nl/frameset.asp?/development/html/publications_db.asp&frnr=1&itemID=1948)

  • Hsu E (2006) The history of qing hao in the Chinese materia medica. Trans R Soc Trop Med Hyg 100:505–508

    Article  PubMed  CAS  Google Scholar 

  • Jones-Brando L, D’Angelo J, Posner GH, Yolken R (2006) In vitro inhibition of Toxoplasma gondii by four new derivatives of artemisinin. Antimicrob Agents Chemother 50:4206–4208

    Article  PubMed  CAS  Google Scholar 

  • Kappe SHI, Vaughan AM, Boddey JA, Cowman AF (2010) That was then but this is now: malaria research in the time of an eradication agenda. Science 328:862–866. doi:10.1126/science.1184785

    Article  PubMed  CAS  Google Scholar 

  • Kumar S, Gupta SK, Singh P, Bajpai P, Gupta MM, Singh D, Gupta AK, Ram G, Shasany AK, Sharma S (2004) High yields of artemisinin by multi-harvest of A. annua crops. Ind Crops Prod 19:77–90

    Article  CAS  Google Scholar 

  • Lévesque F, Seeberger PH (2012) Continuous-flow synthesis of the anti-malaria drug artemisinin. Angew Chem Int Ed 51:1706–1709. doi:10.1002/anie.201107446

    Article  Google Scholar 

  • Lai H, Singh NP (1995) Selective cancer cell cytotoxicity from exposure in dihydroartemisinin and holotransferrin. Cancer Lett 91:41–46

    Article  PubMed  CAS  Google Scholar 

  • Liu B, Wang H, Du Z, Li G, Ye H (2011) Metabolic engineering of artemisinin biosynthesis in A. annua L. Plant Cell Rep 30:689–694

    Article  PubMed  CAS  Google Scholar 

  • Liu KCSC, Yang SL, Roberts MF, Elford BC, Phillipson JD (1992) Antimalarial activity of A. annua flavonoids from whole plants and cell-cultures. Plant Cell Rep 11:637–640

    Article  PubMed  CAS  Google Scholar 

  • Lubbe A, Seibert I, Klimkait T, van der Kooya F (2012) Ethnopharmacology in overdrive: The remarkable anti-HIV activity of Artemisia annua. J Ethnopharmacol 14:854–849. doi: 10.1016/j.jep.2012.03.024

    Google Scholar 

  • Ma Y, Lu D, Lu X, Liao L, Hu X (2004) Activity of dihydroartemisinin against Leishmania donovani both in vitro and vivo. Chin Med J 117:1271–1273

    PubMed  CAS  Google Scholar 

  • Mannan A, Ahmed I, Arshad W, Asim MF, Qureshi RA, Hussain I, Mirza B (2010) Survey of artemisinin production by diverse Artemisia species in northern Pakistan. Malaria J 9:310. doi: 10.1186/1475-2875-9-310

    Article  Google Scholar 

  • Martin VJ, Pitera DJ, Withers ST, Newman JD, Keasling JD (2003) Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol 21:796–802

    Article  PubMed  CAS  Google Scholar 

  • Merali S, Meshnick SR (1991) Susceptibility of Pneumocystis carinii to artemisinin in vitro. Antimicrob Agents Chemother 35:1225–1227

    Article  PubMed  CAS  Google Scholar 

  • Paddon CJ, Westfall PJ, Pitera DJ, Benjamin K, Fisher K, McPhee D, et al (2013) High-level semi-synthetic production of the potent antimalarial artemisinin. Nature 496:528–532

    Google Scholar 

  • Raskin I, Ribnicky DM, Komarnytsky S, Ilic N, Poulev A, Borisjuk N, Brinker A, Moreno DA, Ripoll C, Yakoby N, O’Neal JM, Cornwell T, Pastor I, Fridlender B (2002) Plants and human health in the twenty-first century. Trends Biotechnol 20:522–531

    Article  PubMed  CAS  Google Scholar 

  • Rath K, Taxis K, Walz G, Gleiter CH, Li S-M, Heide L (2004) Pharmacokinetic study of Artemisinin after oral intake of a traditional preparation of Artemisia annua L. (Annualwormwood). Am J Trop Med Hyg 70:128–132

    Google Scholar 

  • Rinaldi A (2004) Fighting malaria at the crossroads: the tools to battle the disease exist, but the lack of political will in developed nations jeopardizes their success. EMBO Rep 5:847–851

    Article  PubMed  CAS  Google Scholar 

  • Ro DK, Paradise EM, Ouellet M, Fisher KJ, Newman KL, Ndungu JM, Ho KA, Eachus RA, Ham TS, Kirby J, Chang MC, Withers ST, Shiba Y, Sarpong R, Keasling JD (2006) Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature 440:940–943

    Article  PubMed  CAS  Google Scholar 

  • Romero MR, Serrano MA, Vallejo M, Efferth T, Alvarez M, Marin JJG (2006) Antiviral effect of artemisinin from A. annua against a model member of the flaviviridae family, the bovine viral diarrhoea virus (BVDV). Planta Med 72:1169–1174

    Article  PubMed  CAS  Google Scholar 

  • Roth RJ, Acton N (1989) A simple conversion of artemisinic acid into artemisinin. J Nat Prod 52:1183–1185

    Article  PubMed  CAS  Google Scholar 

  • Sachs J, Malaney P (2002) The economic and social burden of malaria. Nature 415:680–685

    Article  PubMed  CAS  Google Scholar 

  • Sadava D, Philips T, Lin C (2002) Transferrin overcomes drug resistance to artemisinin in human small cell lung carcinoma cells. Cancer Lett 179:151–156

    Article  PubMed  CAS  Google Scholar 

  • Sangwan NS, Sangwan RS, Kumar S (1998) Isolation of genomic DNA from the antimalarial plant A. annua. Plant Mol Biol Rep 16:1–8

    Article  Google Scholar 

  • Schmid G, Hofheinz W (1983) Total synthesis of qinghaosu. J Am Chem Soc 105:624–625

    Article  CAS  Google Scholar 

  • Singh ND, Lai H (2001) Selective toxicity of dihydroartemisinin and holotransferrin towards human cancer cells. Life Sci 10:49–56

    Article  Google Scholar 

  • Tang HQ, Hu J, Yang L, Tan RX (2000) Terpenoids and flavonoids from Artemisia species. Planta Med 66:391–393

    Article  PubMed  CAS  Google Scholar 

  • Tellez MR, Canel C, Rimando AM, Duke SO (1999) Differential accumulation of isoprenoids in glanded and glandless A. annua L. Photochemistry 52:1035–1040

    Article  CAS  Google Scholar 

  • Teoh KH, Polichuk DR, Reed DW, Nowak G, Covello PS (2006) Artemisia annua L. (Asteraceae) trichome-specific cDNA reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin. FEBS Lett 580:1411–1416

    Article  PubMed  CAS  Google Scholar 

  • Townsend T, Segura V, Chigeza G, Penfield T, Rae A, Harvey D, Bowles D, Graham IA (2013) The use of combining ability analysis to identify elite parents for A. annua F1 hybrid production. PLoS ONE 8(4):e61989. doi:10.1371/journal.pone.0061989

    Article  PubMed  CAS  Google Scholar 

  • Turner KE, Ferreira J F S (2005) Potential use of A. annua in meat goat production systems. In: Cassida K (ed) The conference of the American forage and grassland council, 11–15 June, AFGC, Bloomington, IL, pp 221–225

    Google Scholar 

  • Utzinger T, Shuhua X, Keiser J, Minggan C, Jiang Z, Tanner M (2001) Current progress in the development and use of artemether for chemoprophylaxis of major human schistosome parasites. Curr Med Chem 8:1841–1859

    Article  PubMed  CAS  Google Scholar 

  • Van der Kooy F, Verpoorte R (2011) The content of artemisinin in the A. annua tea infusion. Planta Med 77:1754–1756

    Article  PubMed  Google Scholar 

  • Van Herpen TWJM, Cankar K, Nogueira M, Bosch D, Bouwmeester HJ, Beekwilder J (2010) Nicotiana benthamiana as a production platform for artemisinin precursors. PLoS ONE 5(12):e14222. doi:10.1371/journal.pone.0014222

    Article  PubMed  Google Scholar 

  • Wallaart TE, Bouwmeester HJ, Hille J, Poppinga L, Maijers NC (2001) Amorpha-4,11-diene synthase: cloning and functional expression of a key enzyme in the biosynthetic pathway of the novel antimalarial drug artemisinin. Planta 212:460–465

    Article  PubMed  CAS  Google Scholar 

  • Wallaart TE, Pras N, Quax WJ (1999) Seasonal variations of artemisinin and its biosynthetic precursors in tetraploid A. annua plants compared with the wild-type. Planta Med 65:723–728

    Article  CAS  Google Scholar 

  • Wang J-X, Tang W, Zhou R, Wan J, Shi L-P, Zhang Y, Yang Y-F, Li Y, Zuo J-P (2008) The new water-soluble artemisinin derivative SM905 ameliorates collagen-induced arthritis by suppression of inflammatory and Th17 responses. Br J Pharmacol 153:1303–1310

    Article  PubMed  CAS  Google Scholar 

  • Weathers PJ, Arsenault PR, Covello PS, McMickle A, Teoh KH, Reed DR (2011) Artemisinin production in A. annua: studies in planta and results of a novel delivery method for treating malaria and other neglected diseases. Phytochem Rev 10:173–183. doi:10.1007/s11101-010-9166-0

    Article  PubMed  CAS  Google Scholar 

  • Willcox M, Falquet J, Ferreira JFS, Gilbert B, Hsu E, Melillo de Magalhães P, Plaizier-Vercammen J, Sharma VP, Wright CW (2007) Artemisia annua as a herbal tea for malaria. Afr J Trad Complement Alternat Med 4:121–123

    Google Scholar 

  • Willcox M (2009) Artemisia species: from traditional medicines to modern antimalarials–and back again. J Altern Complement Med 15:101–109

    Article  PubMed  Google Scholar 

  • World Health Organization (2006) Facts on ACTs, Jan 2006 update. WHO, Geneva, Switzerland

    Google Scholar 

  • World Health Organization (2002) Meeting on antimalarial drug development. WHO technical report RS/2001/GE/33 (CHN)

    Google Scholar 

  • Xiao SH, Chollet J, Utzinger J, Matile H, Jinyan M, Tanner M (2001) Artemether administered together with haemin damages schistosomes in vitro. Trans R Soc Trop Med Hyg 95:67–71

    Article  PubMed  CAS  Google Scholar 

  • Xu XX, Zhu J, Huang DZ, Zhou WS (1986) Total synthesis of arteannuin and deoxyarteannuin. Tetrahedron 42:819–828

    Article  CAS  Google Scholar 

  • Yang DM, Liew FY (1993) Effects of qinghaosu (artemisinin) and its derivatives on experimental cutaneous leishmaniasis. Parasitology 106:7–11

    Google Scholar 

  • Zhang Y, Sun H (2009) Immunosuppressive effect of ethanol extract of Artemisia annua on specific antibody and cellular responses of mice against ovalbumin. Immunopharmacol Immunotoxicol 31:625–630

    Article  PubMed  Google Scholar 

  • Zhu C, Cook SP (2012) A concise synthesis of (+)-artemisinin. J Am Chem Soc 134:13577–13579

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Genetics and Biotechnology, University of Calabar, Calabar, Nigeria

    Ebiamadon Andi Brisibe & Peter Nkachukwu Chukwurah

  2. Department of Biological Sciences, Niger Delta University, Amassoma, P.M.B. 71, Yenagoa, Nigeria

    Ebiamadon Andi Brisibe

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  1. Ebiamadon Andi Brisibe
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  2. Peter Nkachukwu Chukwurah
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Correspondence to Ebiamadon Andi Brisibe .

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Editors and Affiliations

  1. Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany

    Tariq Aftab

  2. US Salinity Laboratory, United States Department of Agriculture Agriculture Research Service, Riverside, California, USA

    Jorge F.S. Ferreira

  3. Botany Department, Aligarh Muslim University, Aligarh, India

    M. Masroor A. Khan

  4. Botany Department, Aligarh Muslim University, Aligarh, India

    M. Naeem

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Brisibe, E.A., Chukwurah, P.N. (2014). Production of Artemisinin In Planta and in Microbial Systems Need Not Be Mutually Exclusive. In: Aftab, T., Ferreira, J., Khan, M., Naeem, M. (eds) Artemisia annua - Pharmacology and Biotechnology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-41027-7_15

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