Abstract
Interleukin 4 (IL-4) is a T-cell derived lymphokine that was first described in the murine system as a factor that could costimulate the growth and differentiation of B lymphocytes, but it has subsequently been demonstrated to exhibit pleiotropic effects on various cell types (see [1–3] for reviews). However, detailed characterization of the pharmacological and physicochemical properties of IL-4 has been difficult due to the extremely small quantities available from natural sources. The isolation of cDNA clones for both murine and human IL-4 has permitted the expression of recombinant IL-4 in various types of hosts and subsequent large-scale production of the recombinant cytokines [4, 5]. Similar to its murine analog, recombinant human IL-4 (rhulL-4) has demonstrated pleiotropic effects on both hematopoietic and nonhematopoietic cells and, based on its interesting biological properties, is currently being evaluated in clinical trials in a variety of indications
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Paul WE (1991) Interleukin 4: a prototypic immunoregulatory lymphokine Blood 77: 1859–1870
Banchereau JE (1991) Interleukin 4 and its receptor. In: Mertelsman R (ed) Hematopoietic growth factors in clinical applications. Dekker, New York
Trotta PP (1991) Role of alpha-2 interferon and interleukin 4 in cancer therapy. Am J Reprod Immunol 25: 124–128
Yokota T, Ohtsuka T, Mosmann T, Banchereau J, Defrance T, Blanchard D, de Vries JE, Lee F, Arai K (1986) Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell and T cell-stimulatory activities. Proc Natl Acad Sci USA 83: 5894–5898
Lee F, Yokota T, Ohtsuka J, Meyerson P, Vilaret D, Coffman R, Mosmann T, Rennick D, Roehm N, Smith C, Zlotnick A, Arai K (1986) Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T cell-and mastcell-stimulating activities. Proc Natl Acad Sci USA 83: 2061–2065
Le HV, Ramanathan L, Labdon JE, Mays-Ichinco CA, Syto R, Arai N, Hoy P, Takebe Y, Nagabhushan TL, Trotta PP (1988) Isolation and characterization of multiple variants of recombinant human interleukin 4 expressed in mammalian cells. J Biol Chem 263: 10817–10823
Van Kimmenade A, Bond MW, Schumacher JH, Laquoi C, Kastelein RA (1988) Expression, renaturation and purification of recombinant human interleukin 4 from Escherichia coli. Eur J Biochem 173: 109–114
Her GR, Tsarbopoulos A, Kumarasamy R, Das PP, Pramanik BN, Nagabhushan TL, Trotta PP, Tindall SH (1989) Structural characterization of the recombinant human interleukin 4 N-linked carbohydrate chains. 3 Symposium of the Protein Society, S17
Windsor W, Syto R, Le HV, Trotta PP (1991) Analysis of the conformation and stability of E. coli-derived recombinant human interleukin 4 by circular dichroism. Biochemistry 30: 1259–1264
Windsor WT, Syto R, Durkin J, Das P, Reichert P, Pramanik B, Tindall S, Le HV, Labdon J, Nagabhushan TL, Trotta PP (1990) Disulfide bond assignment of mammalian cell-derived recombinant human interleukin 4. Biophys J 57: 423a
Cook WJ, Ealick SE, Reichert P, Hammond GS, Le HV, Nagabhushan TL, Trotta PP, Bugg CE (1991) Crystallization and preliminary X-ray investigation of recombinant human interleukin 4. J Mol Biol 218: 675–678
Cabrillat H, Galizzi J-P, Djossou O, Arai N, Yokota T, Arai K, Banchereau J (1987) High affinity binding of human interleukin 4 to cell lines. Biochem Biophys Res Commun 149: 995–1001
Idzerda RL, March CJ, Mosley B, Lyman SD, Vanden Bos T, Gimpel SD, Din WS, Grabstein KH, Widmer MB, Park LS, Cosman D, Beckmann MP (1990) Human interleukin 4 receptor confers biological responsiveness and defines a novel receptor superfamily. J Exp Med 171: 861–873
Sullivan L, Bober M, Grace A, Anglin L, Errichetti L, Macosko H, Payvandi F, Sivo C, Waters T, Narula S (1991) A murine system to study the in vivo effect of recombinant cytokines. FASEB J 5: A1613
Plunkett ML, Robertson NE, Halley JA, Downs EC, Catino JJ (1991) Murine interleukin 4 exhibits anti-tumor activity in vivo. Cancer Res (submitted)
Tepper RI, Pattengale PK, Leder P (1989) Murine interleukin-4 displays potent anti-tumor activity in vivo. Cell 57: 503–513
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer-Verlag, Berlin Heidelberg
About this paper
Cite this paper
Trotta, P.P. (1992). Physicochemical and Pharmacological Characterization of Interleukin-4: Implications for Clinical Utility. In: Freund, M., Link, H., Schmidt, R.E., Welte, K. (eds) Cytokines in Hemopoiesis, Oncology, and AIDS II. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-48715-6_3
Download citation
DOI: https://doi.org/10.1007/978-3-642-48715-6_3
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-55242-0
Online ISBN: 978-3-642-48715-6
eBook Packages: Springer Book Archive