Abstract
Here we describe techniques that we developed for monitoring membrane fluidity of individual yeast cells during environmental adaptation and physiological changes. Multi-photon scanning fluorescence microscopy using laurdan as a membrane probe enables determination whether fluidity changes seen by spectroscopy reflect universal responses or changes only of subpopulations.
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Learmonth, R.P., Gratton, E. (2002). Assessment of Membrane Fluidity in Individual Yeast Cells by Laurdan Generalised Polarisation and Multi-photon Scanning Fluorescence Microscopy. In: Kraayenhof, R., Visser, A.J.W.G., Gerritsen, H.C. (eds) Fluorescence Spectroscopy, Imaging and Probes. Springer Series on Fluorescence, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56067-5_14
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DOI: https://doi.org/10.1007/978-3-642-56067-5_14
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-62732-3
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