Abstract
The spleens of 40 male LEW/Ztl rats, bred under specified pathogen-free (SPF) conditions in the Zentrales Tierlabor of Hannover Medical School, were investigated, when the animals weighed 300 g at an age of about 3 months. The rats were killed by ether anaesthesia and organ specimens were either fixed immediately in Bouin’s solution for 24 h before paraffin embedding, or they were frozen and stored in liquid nitrogen for cryostat histology. In addition, a total of ten LEW rats of the same age were housed together with other animals of unknown derivation under “conventional” conditions without any special hygienic precautions.
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© 2000 Springer-Verlag Berlin Heidelberg
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Steiniger, B., Barth, P. (2000). Materials and Methods. In: Microanatomy and Function of the Spleen. Advances in Anatomy, Embryology and Cell Biology, vol 151. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57088-9_2
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DOI: https://doi.org/10.1007/978-3-642-57088-9_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-66161-0
Online ISBN: 978-3-642-57088-9
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