Abstract
Capillary microinjection is one of the most efficient methods for introducing macromolecules into living mammalian cells (Proctor 1992). Samples can be delivered directly into either the nucleus or the cytoplasm. In synchronized cell cultures, the molecules can be transferred at well defined stages of the cell cycle and modifications of culture conditions are possible, before, during, or after injection. Therefore, microinjection of target specific inhibitors (e.g., antibodies, synthetic peptides, antisense mRNAs) or activators (e.g., proteins, peptides, cDNAs under the control of a constitutive promoter) has been a useful tool to study the in vivo function of proteins in cell proliferation and cell cycle control.
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© 1996 Springer-Verlag Berlin Heidelberg
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Pepperkok, R. (1996). Microinjection and Electroporation of Macromolecules into Live Cells. In: Pagano, M. (eds) Cell Cycle — Materials and Methods. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57783-3_7
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DOI: https://doi.org/10.1007/978-3-642-57783-3_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-58066-9
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