Abstract
Reactive oxygen species (ROS) are generally believed to be involved in the etiology of inflammatory disorders, carcinogenesis, and aging of skin (Darr and Fridovich, 1994). Located at the interface between body and environment, the skin is frequently and directly exposed to chemical oxidants, air-pollutants, ultraviolet (UV) solar radiation UV (ultraviolet) solar radiation, and other environmental stressors generating, or containing, free radicals (Shindo et al, 1994; Thiele et al, 1997a; Thiele et al, 1997d). A comprehensive antioxidant network in the skin is thus crucial in protecting this organ from oxidative stress.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aebi H (1974) Catalase. In: Bergmeyer HU, ed. Methods of enzymatic analysis. New York: Academic Press; 673–684.
Allison LA, Shoup RE (1982) Dual electrode liquid chromatography detector for thiols and disulfides. Anal Chem 55:8–12
Darr D, Fridovich I (1994) Free radicals in cutaneous biology. J Invest Dermatol 102:671–675
Dhariwal KR, Hartzel WO, Levine M (1991) Ascorbic acid and dehydroascorbic acid measurements in human plasma and serum. Am J Clin Nutr 54:712–716
Flohe L, Gunzler WA (1984) Assays of glutathione peroxidase. In: Packer L, ed. Methods Enzymol., vol 105. New York: Academic Press; 114–121.
Flohe L, Otting F (1984) Superoxide dismutase assays. In: Packer L, ed. Methods Enzymol., vol 105. New York: Academic Press; 93–104.
Fuchs J, Huflejt ME, Rothfuss LM, Wilson DS, Carcamo G, Packer L (1989) Acute effects of near ultraviolet and visible light on the cutaneous antioxidant defense system. Photochem Photobiol 50:739–744
Gensler HL, Magdaleno M (1991) Topical vitamin E inhibition of immunosuppression and tumorigenesis induced by ultraviolet radiation. Nutr Cancer 15:97–106
Jurkiewicz BA, Bissett DL, Buettner GR (1995) Effect of topically applied tocopherol on ultraviolet radiation-mediated free radical damage in skin. J Invest Dermatol 104:484–488
Lang JK, Gohil K, Packer L (1986) Simultaneous determination of tocopherols, ubiquinols, and ubiquinones in blood, plasma, tissue homogenates, and subcellular fractions. Anal Biochem 157:106–116
Scharffetter-Kochanek K (1997) Photoaging of the connective tissue of skin: Its prevention and therapy, Vol. 38. San Diego, California, USA; London, England, UK: Academic Press, Inc. (Sies H, ed. Antioxidants in disease mechanisms and therapy).
Shindo Y, Witt E, Han D, Epstein W, Packer L (1994) Enzymic and nonenzymic antioxidants in epidermis and dermis of human skin. J Invest Dermatol 102:122–124
Shindo Y, Witt E, Packer L (1993) Antioxidant defense mechanisms in murine epidermis and dermis and their responses to ultraviolet light. J Invest Dermatol 100:260–265
Thiele JJ, Podda M, Packer L (1997a) Tropospheric ozone: an emerging environmental stress to skin. Biol Chem 378: 1299–1305
Thiele JJ, Traber MG, Podda M, Tsang K, Cross CE, Packer L (1997b) Ozone depletes tocopherols and tocotrienols topically applied to murine Skin. FEBS Lett 401:167–170
Thiele JJ, Traber MG, Polefka TG, Cross CE, Packer LP (1997c) Ozone exposure depletes vitamin E and induces lipid peroxidation in murine stratum corneum. J Invest Dermatol 108:753–757
Thiele JJ, Traber MG, Tsang KG, Cross CE, Packer L (1997d) In vivo exposure to ozone depletes vitamins C and E and induces lipid peroxidation in epidermal layers of murine skin. Free Radic Biol Med 23:385–391
Thiele JJ, Traber MG, Packer L (1998) Depletion of human stratum corneum vitamin E: An early and sensitive in vivo marker of UV-induced photooxidation. J Invest Dermatol 110: 756–761
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Thiele, J.J., Lodge, J.K., Choi, J.H., Packer, L. (1999). Measurement of Antioxidants in Cutaneous Tissues. In: Sternberg, H., Timiras, P.S. (eds) Studies of Aging. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59916-3_2
Download citation
DOI: https://doi.org/10.1007/978-3-642-59916-3_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-64600-6
Online ISBN: 978-3-642-59916-3
eBook Packages: Springer Book Archive